Document Detail


Detection of 11q23/MLL rearrangements in infant leukemias with fluorescence in situ hybridization and molecular analysis.
MedLine Citation:
PMID:  7643616     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytogenetic abnormalities of band 11q23 have been found in more than 50% of infant leukemias regardless of the phenotype. Using probes for the MLL gene at 11q23, MLL rearrangements have been identified in 70-80% of all infant leukemias including virtually all of the cases with 11q23 translocations, as well as cases with apparently normal karyotypes. We reviewed the chromosomal pattern of 26 cases of infant leukemias (12 ALL, 12 AML, two AUL). Eleven had 11q23 translocations, five had other abnormalities, and 10 had a normal karyotype. To determine whether 11q23/MLL rearrangements were present in the leukemia cells of patients with a normal karyotype, we performed FISH and molecular studies of eight of these patients who had adequate material. Three were found to have 11q23/MLL abnormalities, two of them detected by FISH; one ALL case had a t(11;19) (q23;p13.3), and one AML case had a t(11;19) (q23;p13.1). Retrospective review confirmed the presence of the t(11;19) in a small percentage of poor quality metaphase cells in both cases. A rearrangement of the MLL gene was detected by Southern blot analysis of leukemic cells from a third patient with ALL; one cell with a deletion of 11q23 was found on karyotypic review. Therefore, in our series the actual incidence of 11q23 abnormalities in infant leukemias was 54% (14/26): 67% in ALL (8/12) and 50% in AML (6/12). Our findings suggest that most infant leukemias with apparently normal karyotypes that have a molecular rearrangement of the MLL gene are undetected subtle translocations.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
J A Martinez-Climent; M J Thirman; R Espinosa; M M Le Beau; J D Rowley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Leukemia     Volume:  9     ISSN:  0887-6924     ISO Abbreviation:  Leukemia     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-09-20     Completed Date:  1995-09-20     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  8704895     Medline TA:  Leukemia     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1299-304     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Chicago Medical Center, IL 60637, USA.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Aberrations / pathology*
Chromosome Banding
Chromosome Disorders
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 19
DNA-Binding Proteins / genetics*
Female
Humans
In Situ Hybridization, Fluorescence
Infant
Infant, Newborn
Leukemia / pathology*
Male
Myeloid-Lymphoid Leukemia Protein
Proto-Oncogenes*
Transcription Factors*
Translocation, Genetic
Zinc Fingers
Grant Support
ID/Acronym/Agency:
CA-42577/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/MLL protein, human; 0/Transcription Factors; 149025-06-9/Myeloid-Lymphoid Leukemia Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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