Document Detail


Detection of radiation effects using recombinant bioluminescent Escherichia coli strains.
MedLine Citation:
PMID:  10789894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Effects of ionizing radiation (0.1-500 Gy) on recombinant Escherichia coli cells containing the stress promoters recA, grpE, or katG, fused to luxCDABE, were characterized by monitoring transcriptional responses reflected by the bioluminescent output. The minimum dose of gamma-irradiation detected by E. coli DPD2794 (recA::luxCDABE) was about 1.5 Gy, while the maximum response was obtained at 200 Gy. The amount of emitted bioluminescence increased proportionally with the gamma-ray doses which were found to elicit a DNA damage response in a range of 1-50 Gy. In addition, the cell growth rate was severely, but transiently, retarded by about 50 Gy. Quantification of the gamma-ray dose may be possible using the recA promoter fusion, since linear enhancement of the bioluminescence emission with increasing gamma-ray dose was observed. Other irradiated strains (50 Gy) responsive to either oxidative stress (DPD2511, katG::luxCDABE) or protein-damaging stress (TV1061, grpE::luxCDABE) did not display an increased bioluminescent output, while DPD2794 irradiated by the same dose of gamma-rays gave a significant bioluminescent output. This indicates that the recA promoter is the one most suitable for developing a biosensor for ionizing radiation.
Authors:
J Min; C W Lee; S H Moon; R A LaRossa; M B Gu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation and environmental biophysics     Volume:  39     ISSN:  0301-634X     ISO Abbreviation:  Radiat Environ Biophys     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-06-08     Completed Date:  2000-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0415677     Medline TA:  Radiat Environ Biophys     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  41-5     Citation Subset:  IM    
Affiliation:
Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology (K-JIST), Korea.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics
Dose-Response Relationship, Radiation
Escherichia coli / genetics,  growth & development,  radiation effects*
Escherichia coli Proteins*
Gamma Rays
Heat-Shock Proteins / genetics
Luminescent Measurements
Operon
Peroxidases / genetics
Rec A Recombinases / genetics
Recombination, Genetic
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Escherichia coli Proteins; 0/GrpE protein, Bacteria; 0/GrpE protein, E coli; 0/Heat-Shock Proteins; EC 1.11.1.-/Peroxidases; EC 1.11.1.6/catalase HPI; EC 2.7.7.-/Rec A Recombinases

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