| Detection of quinone oxidoreductase 1 (NQO1) single-nucleotide polymorphisms (SNP) related to benzene metabolism in immortalized B lymphocytes from a Chinese Han population. | |
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MedLine Citation:
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PMID: 20391128 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Single-nucleotide polymorphisms (SNP) in genes coding metabolizing enzymes modulate gene functions and cellular toxicity in response to chemicals. Quinone oxidoreductase 1 (NQO1) is an important detoxification enzyme involved in the catabolism of 1,4-benzoquinone (1,4-BQ), a benzene metabolite believed to be associated with bone-marrow toxicity and leukemia. Gene function was evaluated in immortalized human B lymphocytes derived from a Chinese Han population with independent genotypes at 2 NQO1 SNP sites. 1,4-Benzoquinone was incubated with these immortalized lymphocytes of differing genotypes. Among the genotypes of 2 SNP examined, cell lines with rs1800566CC showed a higher NQO1 enzymic activity after a 48 h of treatment with 10 muM 1,4-BQ, and a lower comet rate compared with cells of CT/TT genotypes. Data suggested that NQO1 rs1800566 might serve as a functional genetic marker for benzene toxicity in the Chinese Han population. The immortalized B lymphocytes derived from different populations might thus be used as a biomarker to detect functional genetic markers related to exposure to environmental chemicals. |
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Authors:
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Juan Zhang; Lihong Yin; Geyu Liang; Ran Liu; Yuepu Pu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of toxicology and environmental health. Part A Volume: 73 ISSN: 1528-7394 ISO Abbreviation: J. Toxicol. Environ. Health Part A Publication Date: 2010 |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-06-17 Revised Date: 2012-05-28 |
Medline Journal Info:
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Nlm Unique ID: 100960995 Medline TA: J Toxicol Environ Health A Country: England |
Other Details:
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Languages: eng Pagination: 490-8 Citation Subset: IM |
Affiliation:
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Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult B-Lymphocytes / enzymology*, metabolism Benzene / metabolism*, toxicity Benzoquinones / metabolism, toxicity Cell Line Cell Proliferation / drug effects China / ethnology DNA Damage DNA Fingerprinting Enzyme Induction / drug effects Female Humans Male Middle Aged NAD(P)H Dehydrogenase (Quinone) / genetics*, metabolism Polymorphism, Single Nucleotide* RNA, Messenger / metabolism Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Benzoquinones; 0/RNA, Messenger; 106-51-4/benzoquinone; 71-43-2/Benzene; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone); EC 1.6.5.2/NQO1 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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