Document Detail


Detection of quinone oxidoreductase 1 (NQO1) single-nucleotide polymorphisms (SNP) related to benzene metabolism in immortalized B lymphocytes from a Chinese Han population.
MedLine Citation:
PMID:  20391128     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Single-nucleotide polymorphisms (SNP) in genes coding metabolizing enzymes modulate gene functions and cellular toxicity in response to chemicals. Quinone oxidoreductase 1 (NQO1) is an important detoxification enzyme involved in the catabolism of 1,4-benzoquinone (1,4-BQ), a benzene metabolite believed to be associated with bone-marrow toxicity and leukemia. Gene function was evaluated in immortalized human B lymphocytes derived from a Chinese Han population with independent genotypes at 2 NQO1 SNP sites. 1,4-Benzoquinone was incubated with these immortalized lymphocytes of differing genotypes. Among the genotypes of 2 SNP examined, cell lines with rs1800566CC showed a higher NQO1 enzymic activity after a 48 h of treatment with 10 muM 1,4-BQ, and a lower comet rate compared with cells of CT/TT genotypes. Data suggested that NQO1 rs1800566 might serve as a functional genetic marker for benzene toxicity in the Chinese Han population. The immortalized B lymphocytes derived from different populations might thus be used as a biomarker to detect functional genetic markers related to exposure to environmental chemicals.
Authors:
Juan Zhang; Lihong Yin; Geyu Liang; Ran Liu; Yuepu Pu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of toxicology and environmental health. Part A     Volume:  73     ISSN:  1528-7394     ISO Abbreviation:  J. Toxicol. Environ. Health Part A     Publication Date:  2010  
Date Detail:
Created Date:  2010-04-14     Completed Date:  2010-06-17     Revised Date:  2012-05-28    
Medline Journal Info:
Nlm Unique ID:  100960995     Medline TA:  J Toxicol Environ Health A     Country:  England    
Other Details:
Languages:  eng     Pagination:  490-8     Citation Subset:  IM    
Affiliation:
Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
B-Lymphocytes / enzymology*,  metabolism
Benzene / metabolism*,  toxicity
Benzoquinones / metabolism,  toxicity
Cell Line
Cell Proliferation / drug effects
China / ethnology
DNA Damage
DNA Fingerprinting
Enzyme Induction / drug effects
Female
Humans
Male
Middle Aged
NAD(P)H Dehydrogenase (Quinone) / genetics*,  metabolism
Polymorphism, Single Nucleotide*
RNA, Messenger / metabolism
Young Adult
Chemical
Reg. No./Substance:
0/Benzoquinones; 0/RNA, Messenger; 106-51-4/benzoquinone; 71-43-2/Benzene; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone); EC 1.6.5.2/NQO1 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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