Document Detail


Detection and quantification of four species of the genus Clostridium in infant feces.
MedLine Citation:
PMID:  16301809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine the composition of Clostridium in the feces of infants approximately 30 days old, we have developed a detection and quantification method of Clostridium paraputrificum, Clostridium perfringens, Clostridium tertium, and Clostridium difficile by species-specific primers. C. perfringens and C. difficile were detected in four fecal samples from 22 infants (18.2%), whereas C. paraputrificum was detected in three samples (16.7%). C. tertium was detected in two samples (9.1%). Moreover, the occurrences of the four species in bottle-and mix-fed infants were relatively higher than in breast-fed infants (P< 0.05). Subsequently, positive samples detected by nested PCR (polymerase chain reaction) were subjected to realtime PCR. The results showed that the numbers of C. paraputrificum, C. perfringens, C. tertium, and C. difficile ranged from about 1x10(5) to 3x10(7) cells/g wet feces.
Authors:
Toshiki Tonooka; Shinji Sakata; Maki Kitahara; Michio Hanai; Shinobu Ishizeki; Masaaki Takada; Mitsuo Sakamoto; Yoshimi Benno
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Microbiology and immunology     Volume:  49     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-11-22     Completed Date:  2006-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  987-92     Citation Subset:  IM    
Affiliation:
Wakodo Co., Ltd, Chofu, Tokyo, Japan. BCK00321@nifty.com
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MeSH Terms
Descriptor/Qualifier:
Bottle Feeding
Breast Feeding
Cell Count
Clostridium / genetics,  isolation & purification*
Clostridium difficile / genetics,  isolation & purification
Clostridium perfringens / genetics
Clostridium tertium / genetics,  isolation & purification
DNA Primers
Feasibility Studies
Feces / microbiology*
Female
Humans
Infant
Infant, Newborn
Male
Molecular Diagnostic Techniques*
Polymerase Chain Reaction / methods
Sensitivity and Specificity
Species Specificity
Chemical
Reg. No./Substance:
0/DNA Primers

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