| Detection and quantification of ATP and heavy metal with electronical micro-circuits. | |
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MedLine Citation:
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PMID: 19003313 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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In order to use whole eukaryotic cells as an active element in the detection and amplification of biological signals, for both in vitro and in vivo applications, we have undertaken a first approach to interface live cells and integrated circuit, and evaluate the possibility to develop a microbioreactor. An amplified photodiode system was designed and built as an electronical circuit in a way that it could easily be miniaturised. In parallel micro-chips with silicium chambers were used as microbioreactors to adhere cells. We showed here that this etched silicon chamber allows endothelial and CHO cells spreading, permitting determination of a number of cell properties {\it on line} providing appropriate integrated circuits are designed to perform the desired functions. The photodiode system reacting to the luminescent luciferase system permitted, through the use of appropriate software from a personal computer (PC) connected on line in vitro, the determination of ATP concentration, and using different luciferase transfected bacteria permitted the detection of constitutive or induced luminescence. |
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Authors:
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V Hendrick; O Charlier; B Mommens; M J Goffaux; T Marique; L De Vos; J Wérenne |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cytotechnology Volume: 36 ISSN: 0920-9069 ISO Abbreviation: Cytotechnology Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2008-11-12 Completed Date: 2011-07-14 Revised Date: 2013-03-05 |
Medline Journal Info:
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Nlm Unique ID: 8807027 Medline TA: Cytotechnology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 41-7 Citation Subset: - |
Affiliation:
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Animal Cell Biotechnology (, Université Libre de Bruxelles, C.P. 160/17, Brussels, Belgium, vincianne.hendrick@compaqnet.be. |
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