Document Detail


Detection of protease activity with a fluorescence-labelled peptide substrate on a TLC plate.
MedLine Citation:
PMID:  10923804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A small amount of peptidase activity could be detected using an amine derivatizing reagent, fluorescein isothiocyanate (FITC), which has been used to produce a fluorogenic peptide. The substrate produced, FITC-peptide, gave a clear spot on a silica gel sheet upon exposure to UV light. The peptidase activity of angiotensin-converting enzyme (ACE), trypsin, chymotrypsin, cucumisin, and that of some plant tissues were detected by using a fluorogenic angiotensin I. This showed that the substrate specificity of proteolytic enzymes can be distinguished from the others by this procedure.
Authors:
T Uchikoba; H Yonezawa; K Arima; M Kaneda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  64     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-12-18     Completed Date:  2000-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1285-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Faculty of Science, Kagoshima University, Korimoto, Japan. uchik@sci.kagoshima-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Angiotensin I
Animals
Chromatography, Thin Layer
Chymotrypsin / analysis
Endopeptidases / analysis*
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Humans
Peptidyl-Dipeptidase A / analysis
Plants / enzymology
Serine Endopeptidases / analysis
Trypsin / analysis
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 3326-32-7/Fluorescein-5-isothiocyanate; 9041-90-1/Angiotensin I; EC 3.4.-/Endopeptidases; EC 3.4.15.1/Peptidyl-Dipeptidase A; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.1/Chymotrypsin; EC 3.4.21.25/cucumisin; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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