Document Detail


Detection of prostaglandin E2-induced dendritic cell migration into the lymph nodes of mice using a 1.5 T clinical MR scanner.
MedLine Citation:
PMID:  22009917     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The control of dendritic cell (DC) migration into lymph nodes (LNs) is important for the development of more effective DC-based immunotherapies. This study was undertaken to evaluate, dynamically and noninvasively, prostaglandin E2 (PGE2)-enhanced migration of DCs using a 1.5 T clinical MR scanner. DC2.4 cells were labeled with superparamagnetic iron oxide (SPIO), a clinically approved MRI contrast agent. DCs were stimulated with tumor necrosis factor-α and interferon-γ in the presence or absence of PGE2. Before and after subcutaneous injection of labeled DCs into the hind leg footpads of mice, MRI detailing the extent of DC migration into popliteal LNs was performed using a 1.5 T clinical MR scanner. SPIO labeling did not influence the viability, endocytic activity, migratory ability and/or co-stimulatory molecule expression of DCs. PGE2 enhanced significantly chemokine receptor-7 expression and the migration of DCs (p < 0.05). After subcutaneous injection of DCs, there were decreases in MR signal intensity in popliteal LNs at 24 h post-injection; in PGE2-treated cells, the MR signal intensity was significantly lower (decrease of 86.6 ± 2.5%) than in PGE2-untreated cells (decrease of 70.0 ± 4.2%) (p < 0.05). Histological analyses with the conventionally used Prussian blue stain demonstrated that the PGE2-treated DCs migrated more deeply into the center of LNs. PGE2-enhanced migration of SPIO-labeled DCs into LNs can be detected using a 1.5 T clinical MR scanner. Our results suggest that in vivo MRI of DC migration is a useful imaging method to predict DC therapy with a high rate of efficacy and to improve DC-based immunotherapy, thereby reducing costs compared with current treatments in clinical trials. Copyright © 2011 John Wiley & Sons, Ltd.
Authors:
Hyun Jung Joo; Hoe Suk Kim; Yoon Seok Choi; Hyeonjin Kim; Seung Ja Kim; Woo Kyung Moon
Related Documents :
20663967 - Pulmonary emphysema: effect of lung volume on objective quantification at thin-section ct.
12764637 - On visual determination of full inspiration on ct images.
24677597 - Mri of hepatic epithelioid hemangioendothelioma (heh).
23411717 - Preoperative detection of the facial nerve by high-field magnetic resonance imaging in ...
18766427 - Vertebral "clover" scintigraphic image in a vertebral metastasis misdiagnosed with page...
22405857 - Discitis and sacroiliitis diagnosed 15years after iatrogenic mycobacterium xenopi inocu...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-18
Journal Detail:
Title:  NMR in biomedicine     Volume:  -     ISSN:  1099-1492     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8915233     Medline TA:  NMR Biomed     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 John Wiley & Sons, Ltd.
Affiliation:
Department of Radiology, Seoul National University Hospital, Seoul, South Korea; Department of Biomedical Science, College of Medicine, Seoul National University, Seoul, South Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Carotenoids in Age-related Maculopathy Italian Study (CARMIS): two-year results of a randomized stud...
Next Document:  Measurement of Hearing Protection Devices Performance in the Workplace during Full-Shift Working Ope...