| Detection of a progression-linked DNA restriction fragment in rat hepatoma cells probed with a hexokinase cDNA. | |
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MedLine Citation:
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PMID: 8094714 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have compared genomic DNA isolated from a series of rat hepatomas and from normal rat liver tissue by restriction fragment analysis. Our objective was to identify a tumor-specific restriction fragment that would be present in rapidly growing tumors and missing in normal tissues. Using a cDNA probe specific for the C-terminal half of rat brain hexokinase, we have identified several changes in DNA restriction fragment lengths in rat hepatomas vs. normal rat liver. Most of these involved restriction fragments that were present in normal rat DNA and missing in tumor DNA. However, one HinfI fragment of M(r) = 13.4 kilobase pairs (kb) was found to be present in rapidly growing rat hepatomas, but missing in normal rat tissue and in hepatomas of slow or intermediate growth rate. |
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Authors:
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W C Wigley; G E Corson; R A Nakashima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 53 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 1993 Feb |
Date Detail:
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Created Date: 1993-03-23 Completed Date: 1993-03-23 Revised Date: 2007-07-24 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 657-61 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA / genetics DNA Probes DNA, Neoplasm / genetics Hexokinase / genetics* Liver Neoplasms, Experimental / genetics*, pathology Polymorphism, Restriction Fragment Length Rats Rats, Inbred Strains Rats, Sprague-Dawley Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/DNA, Neoplasm; 9007-49-2/DNA; EC 2.7.1.1/Hexokinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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