| Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication. | |
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MedLine Citation:
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PMID: 22098822 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A commercially available real-time PCR, based on a multi-copy target cytochrome b (cyt b) using porcine specific primers, has been validated for the Halal/Kosher authentication of gelatine. Extraction and purification of DNA from gelatine were successfully achieved using the SureFood® PREP Animal system, and real-time PCR was carried out using SureFood® Animal ID Pork Sens kit. The minimum level of adulteration that could be detected was 1.0% w/w for marshmallows and gum drops. A small survey was undertaken of processed food products such as gum drops, marshmallows and Turkish delight, believed to contain gelatine. Of fourteen food products from Germany, two samples were found to contain porcine gelatine, whereas of twenty-nine samples from Turkey twenty-eight were negative. However, one product from Turkey contained porcine DNA and thus was not Halal, and neither was the use of porcine gelatine indicated on the product label. |
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Authors:
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Yasemin Demirhan; Pelin Ulca; Hamide Z Senyuva |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-31 |
Journal Detail:
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Title: Meat science Volume: - ISSN: 1873-4138 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-11-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101160862 Medline TA: Meat Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
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A&T Food Laboratory, Mega Center No. 29 34045, Istanbul, Turkey. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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