Document Detail


Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues.
MedLine Citation:
PMID:  17505669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Parvovirus B19 infection was first discovered in 1975 and it is implicated in fetal death from hydrops fetalis the world over. Diagnosis is usually made through histological identification of the intranuclear inclusion in placenta and fetal organs. However, these cells may be scarce or uncharacteristic, making definitive diagnosis difficult. We analyzed histologically placentas and fetal organs from 34 cases of non-immune hydrops fetalis, stained with Hematoxylin and Eosin (HE) and submitted to immunohistochemistry and polymerase chain reaction (PCR). Of 34 tissue samples, two (5.9%) presented typical intranuclear inclusion in circulating normoblasts seen in Hematoxylin and Eosin stained sections, confirmed by immunohistochemistry and PCR. However, PCR of fetal organs was negative in one case in which the placenta PCR was positive. We concluded that parvovirus B19 infection frequency is similar to the literature and that immunohistochemistry was the best detection method. It is highly specific and sensitive, preserves the morphology and reveals a larger number of positive cells than does HE with the advantage of showing cytoplasmic and nuclear positivity, making it more reliable. Although PCR is more specific and sensitive in fresh or ideally fixed material it is not so in formalin-fixed paraffin-embedded tissues, frequently the only one available in such cases.
Authors:
Paulo Roberto Veiga Quemelo; Danielle Malta Lima; Benedito Antônio Lopes da Fonseca; Luiz Cesar Peres
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Revista do Instituto de Medicina Tropical de São Paulo     Volume:  49     ISSN:  0036-4665     ISO Abbreviation:  Rev. Inst. Med. Trop. Sao Paulo     Publication Date:    2007 Mar-Apr
Date Detail:
Created Date:  2007-05-16     Completed Date:  2007-07-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7507484     Medline TA:  Rev Inst Med Trop Sao Paulo     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  103-7     Citation Subset:  IM    
Affiliation:
Department of Pathology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, 14049-900, Brazil. pquemelo@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Viral / analysis
Coloring Agents
Eosine Yellowish-(YS)
Female
Fetus / pathology,  virology*
Hematoxylin
Humans
Hydrops Fetalis / pathology,  virology*
Immunohistochemistry
Male
Paraffin Embedding
Parvoviridae Infections / embryology*,  pathology
Parvovirus B19, Human / genetics,  immunology,  isolation & purification*
Placenta / pathology,  virology*
Polymerase Chain Reaction
Pregnancy
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Coloring Agents; 17372-87-1/Eosine Yellowish-(YS); 517-28-2/Hematoxylin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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