Document Detail

Detection of parvovirus B19 IgM by antibody capture enzyme immunoassay: receiver operating characteristic analysis.
MedLine Citation:
PMID:  11064115     Owner:  NLM     Status:  MEDLINE    
Parvovirus B19 infection can cause severe effects in high-risk groups including pregnant women and immunocompromised individuals. Although serological detection of B19 infection is commonplace, minimal information is available on the absolute performance characteristics of various tests for the detection of B19 IgM. The performance of the first parvovirus B19 IgM enzyme immunoassay to be cleared by the US Food and Drug Administration (FDA) is described. The immunoassay cut-off has been established using receiver operating characteristic (ROC) analysis giving a sensitivity and specificity of detection of 89.1 and 99.4%, respectively. No cross-reactivity is observed with rubella or other viral disease IgM which cause similar symptomologies to parvovirus B19. Multi-site reproducibility studies have shown high immunoassay reproducibility with detection rates (observed/expected result) of 100% for nonreactive specimens (N=324) and strongly reactive (N=403), respectively. Immunoassay reproducibility ranged from 11.76 to 17. 46% coefficient of variation for all reactive specimens tested (N=12) whereby each specimen was assayed a total of 81 times. Parvovirus B19 IgM seroprevalence of 1% was observed in a US blood donor population (N=399). In the absence of international performance criteria, this study will be of major benefit to the clinical virologist in assessing immunoassay reliability for the detection of recent infection with parvovirus B19.
S Doyle; S Kerr; G O'Keeffe; D O'Carroll; P Daly; C Kilty
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virological methods     Volume:  90     ISSN:  0166-0934     ISO Abbreviation:  J. Virol. Methods     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-04     Completed Date:  2000-12-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8005839     Medline TA:  J Virol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  143-52     Citation Subset:  IM    
Department of Biology, National University of Ireland, Co. Kildare, Maynooth, Ireland.
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MeSH Terms
Antibodies, Viral / blood*
Blood Donors
Cross Reactions
Immunoenzyme Techniques / methods,  standards*
Immunoglobulin M / blood*
Parvoviridae Infections / epidemiology,  virology*
Parvovirus B19, Human / immunology*
ROC Curve
Reproducibility of Results
Seroepidemiologic Studies
United States / epidemiology
Reg. No./Substance:
0/Antibodies, Viral; 0/Immunoglobulin M

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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