Document Detail

Detection of non-diploid cells in premalignant and malignant oral lesions using combined morphological and FISH analysis - a new method for early detection of suspicious oral lesions.
MedLine Citation:
PMID:  17386971     Owner:  NLM     Status:  MEDLINE    
Alteration in DNA content is an early event in oral carcinogenesis. We have examined oral brush samples to detect non-diploid cells (NDC) using simultaneous morphological and cytogenetic analysis. The study included 8 oral squamous cell carcinomas (OSCC), 22 premalignant lesions (OPLs), and 25 control individuals. Slides stained with Giemsa followed by FISH using chromosome 2 centromeric DNA probe, were scanned and fluorescent signals were simultaneously analyzed in parallel with the morphology. The proportion of NDC increased with the severity of the diagnosis. In two control subjects, 1-1.5% of the examined cells were NDC. Over 2% NDC were present in all OSCC cases and in 11 of the OPLs, of which, in 8 the histologic diagnosis was either epithelial hyperplasia or mild dysplasia. A significant number of NDC had normal morphology when cytomorphology and FISH were compared. Two patients with OPLs developed OSCC these patients had a significant proportion of NDC. We suggest that the combined morphological and cytogenetic analysis of cells collected by a non-invasive brush sampling can enhance early detection of potentially malignant cells.
Abraham Hirshberg; Noam Yarom; Ninette Amariglio; Ran Yahalom; Iris Adam; Rachel Stanchescu; Issahar Ben-Dov; Shlomo Taicher; Gideon Rechavi; Luba Trakhtenbrot
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-26
Journal Detail:
Title:  Cancer letters     Volume:  253     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-09     Completed Date:  2007-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  282-90     Citation Subset:  IM    
Department of Oral Pathology and Oral Medicine, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
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MeSH Terms
Aged, 80 and over
Chromosomes, Human, Pair 2 / genetics
Cytogenetic Analysis / methods*
DNA, Neoplasm / analysis
Early Diagnosis
In Situ Hybridization, Fluorescence / methods*
Middle Aged
Mouth Neoplasms / diagnosis*,  genetics,  pathology
Neoplasms, Squamous Cell / diagnosis*,  genetics,  pathology
Precancerous Conditions / diagnosis*,  genetics,  pathology
Reg. No./Substance:
0/DNA, Neoplasm

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