| Detection of non-diploid cells in premalignant and malignant oral lesions using combined morphological and FISH analysis - a new method for early detection of suspicious oral lesions. | |
| | |
MedLine Citation:
|
PMID: 17386971 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Alteration in DNA content is an early event in oral carcinogenesis. We have examined oral brush samples to detect non-diploid cells (NDC) using simultaneous morphological and cytogenetic analysis. The study included 8 oral squamous cell carcinomas (OSCC), 22 premalignant lesions (OPLs), and 25 control individuals. Slides stained with Giemsa followed by FISH using chromosome 2 centromeric DNA probe, were scanned and fluorescent signals were simultaneously analyzed in parallel with the morphology. The proportion of NDC increased with the severity of the diagnosis. In two control subjects, 1-1.5% of the examined cells were NDC. Over 2% NDC were present in all OSCC cases and in 11 of the OPLs, of which, in 8 the histologic diagnosis was either epithelial hyperplasia or mild dysplasia. A significant number of NDC had normal morphology when cytomorphology and FISH were compared. Two patients with OPLs developed OSCC these patients had a significant proportion of NDC. We suggest that the combined morphological and cytogenetic analysis of cells collected by a non-invasive brush sampling can enhance early detection of potentially malignant cells. |
| | |
Authors:
|
Abraham Hirshberg; Noam Yarom; Ninette Amariglio; Ran Yahalom; Iris Adam; Rachel Stanchescu; Issahar Ben-Dov; Shlomo Taicher; Gideon Rechavi; Luba Trakhtenbrot |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-26 |
Journal Detail:
|
Title: Cancer letters Volume: 253 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2007 Aug |
Date Detail:
|
Created Date: 2007-07-09 Completed Date: 2007-10-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
|
Languages: eng Pagination: 282-90 Citation Subset: IM |
Affiliation:
|
Department of Oral Pathology and Oral Medicine, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel. hirshmd@post.tau.ac.il |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Aged, 80 and over Chromosomes, Human, Pair 2 / genetics Cytogenetic Analysis / methods* DNA, Neoplasm / analysis Diploidy Early Diagnosis Female Humans In Situ Hybridization, Fluorescence / methods* Male Middle Aged Mouth Neoplasms / diagnosis*, genetics, pathology Neoplasms, Squamous Cell / diagnosis*, genetics, pathology Precancerous Conditions / diagnosis*, genetics, pathology |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Neoplasm |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Human chorionic gonadotropin (hCG) and prevention of breast cancer.
Next Document: Adsorption characteristics of Cu(II) and Pb(II) onto expanded perlite from aqueous solution.