| Detection of nitric oxide and its derivatives in human mixed saliva and acidified saliva. | |
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MedLine Citation:
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PMID: 18423231 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nitrate is secreted into the human oral cavity as a salivary component. The nitrate is transformed to nitrite and nitric oxide (NO) by oral bacteria. NO is oxidized by O(2) producing NO(2) and N(2)O(3) and also by O(2)(-) producing ONOO(-). Salivary peroxidase can oxidize nitrite and NO to NO(2) or its equivalent in the oral cavity. Nitrite dissolved in saliva is mixed with gastric juice, generating nitrous acid that is transformed to NO and NO(2) via N(2)O(3) by self-decomposition. In addition, nitrous acid can react with ascorbic acid and phenolics producing NO and with H(2)O(2) producing ONOOH. This chapter deals with the detection of reactive nitrogen oxide species (RNOS), especially NO, N(2)O(3), NO(2), and ONOO(-)/ONOOH, in mixed whole saliva and acidified saliva using fluorescent probes and spin-trapping reagents. It is also shown that measurements of nitration and oxygen consumption are useful in studying the formation and scavenging of RNOS in the aforementioned systems. |
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Authors:
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Umeo Takahama; Sachiko Hirota; Oniki Takayuki |
Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Methods in enzymology Volume: 440 ISSN: 0076-6879 ISO Abbreviation: Meth. Enzymol. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-04-21 Completed Date: 2008-06-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 381-96 Citation Subset: IM |
Affiliation:
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Department of Bioscience, Kyushu Dental College, Kitakyushu, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Humans Hydrogen-Ion Concentration Nitric Oxide / analogs & derivatives*, analysis*, chemistry, metabolism Reactive Oxygen Species / chemistry, metabolism Saliva / chemistry*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 10102-43-9/Nitric Oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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