Document Detail


Detection of nine microorganisms from the initial carious root lesions using a TaqMan-based real-time PCR.
MedLine Citation:
PMID:  21605286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The purpose of this study was to quantify nine selected cariogenic bacteria in plaque from sound root surfaces and initial carious root lesions using TaqMan PCR and to analyse a putative dependence on the kind of initial periodontal treatment.
MATERIAL AND METHODS: Fifty-four subjects with generalized chronic periodontitis were randomly allocated to one of the following initial periodontal therapies: full-mouth disinfection, full-mouth scaling and root planing or scaling and root planing within 7 days. Plaque samples were taken before and after periodontal treatment and analysed by TaqMan PCR.
RESULTS: The quantity of the cariogenic bacteria Actinomyces spp., Streptococcus mutans, Streptococcus sobrinus, Lactobacilllus spp., Rothia dentocariosa, Parvimonas micra, Propionibacterium acnes and Neisseria mucosa were significantly higher, while the quantity of Veillonella parvula was significantly lower on initial carious lesions than on the sound surfaces both before and after periodontal therapy. No significant differences could be found in any of the tested bacteria except P. micra on initial carious lesions and sound surfaces for both examinations between the groups.
CONCLUSION: All the nine species analysed were found to be present in initial carious root lesions as well as sound root surfaces but in different quantities, independent of the different periodontal therapies.
Authors:
M Bizhang; Bi Ellerbrock; D Preza; Whm Raab; P Singh; T Beikler; B Henrich; S Zimmer
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Publication Detail:
Type:  Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't     Date:  2011-05-24
Journal Detail:
Title:  Oral diseases     Volume:  17     ISSN:  1601-0825     ISO Abbreviation:  Oral Dis     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-09     Completed Date:  2012-01-26     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9508565     Medline TA:  Oral Dis     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  642-52     Citation Subset:  D    
Copyright Information:
© 2011 John Wiley & Sons A/S.
Affiliation:
Department of Operative and Preventive Dentistry, University Witten/Herdecke, Witten, Germany. mozhgan.bizhang@uni-wh.de
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MeSH Terms
Descriptor/Qualifier:
Actinomyces / isolation & purification
Actinomycetaceae / isolation & purification
Anti-Infective Agents, Local / therapeutic use
Bacterial Load
Chlorhexidine / analogs & derivatives,  therapeutic use
Chronic Periodontitis / microbiology,  therapy
DNA Probes
Dental Plaque / microbiology
Dental Scaling
Female
Gram-Negative Bacteria / classification*
Gram-Positive Bacteria / classification*
Humans
Lactobacillus / isolation & purification
Male
Middle Aged
Neisseria mucosa / isolation & purification
Peptostreptococcus / isolation & purification
Propionibacterium acnes / isolation & purification
Real-Time Polymerase Chain Reaction / methods*
Root Caries / microbiology*
Root Planing
Streptococcus mutans / isolation & purification
Streptococcus sobrinus / isolation & purification
Taq Polymerase
Tooth Root / microbiology
Veillonella / isolation & purification
Chemical
Reg. No./Substance:
0/Anti-Infective Agents, Local; 0/DNA Probes; 55-56-1/Chlorhexidine; EC 2.7.7.-/Taq Polymerase; MOR84MUD8E/chlorhexidine gluconate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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