Document Detail


Detection of new allotypic variants of bovine λ-light chain constant regions in different cattle breeds.
MedLine Citation:
PMID:  21741991     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In the cattle breeds German Black Pied (GBP), German Simmental (GS), Holstein Friesian (HF), Aubrac (A) three transcribed allotypic variants in isotype IGLC2 and five allotypic variants in isotype IGLC3 were identified. Substitutions within the putative interface to CH1 at position 11 and 79 were noted. In IGLC2(b), K79E led to a charge conversion. In IGLC3(b) and IGLC3(c), the E79N replacement removed the charge while the T11K substitution resulted in a positively charged amino acid residue. In addition, D15 and T16 were found in IGLC2(c), IGLC3(b), and IGLC3(c). Substitutions located on the outer site of the molecule were observed in IGLC2(b) (V40, H45.5), IGLC2(c) (A1, V40, D77), IGLC3(b) (A1, D77, D109, P127), IGLC3(c) (A1, G45.5, D77, D109, P127), IGLC3(d) (D109), and IGLC3(e) (A1). Amino acid residues P83 (IGLC2(c), IGLC3(b), IGLC3(c)), N93 (IGLC2(b)), D93 (IGLC3(b)), and G93 (IGLC3(c)) were positioned in cavities but seemed to be accessible for solvents.
Authors:
Ulrike S Diesterbeck; Dalia M Aboelhassan; Sina K Stein; Claus-Peter Czerny
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-1
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  -     ISSN:  1879-0089     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ltd.
Affiliation:
Department of Animal Sciences, Institute of Veterinary Medicine, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Sciences, Georg-August University Goettingen, Burckhardtweg 2, 37077 Goettingen, Germany.
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