| Detection of mutations by denaturing gradient gel electrophoresis. | |
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MedLine Citation:
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PMID: 18428306 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This unit describes the procedure for determining the melting profile for a given PCR-amplified sequence by perpendicular denaturing gradient gel electrophoresis (DGGE) and, using that information, for developing a screening assay based on either parallel DGGE, CDGE (Constant Denaturant Gel Electrophoresis), or TTGE (Temporal/Temperature Gradient Electrophoresis). Four support protocols describe techniques for pouring perpendicular and parallel denaturing gradient gels, constant denaturant gels, and temporal temperature gradient gels. |
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Authors:
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A L Børresen-Dale; E Hovig; B Smith-Sørensen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.] Volume: Chapter 7 ISSN: 1934-8258 ISO Abbreviation: - Publication Date: 2001 May |
Date Detail:
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Created Date: 2008-04-22 Completed Date: 2008-05-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101287858 Medline TA: Curr Protoc Hum Genet Country: United States |
Other Details:
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Languages: eng Pagination: Unit 7.5 Citation Subset: IM |
Affiliation:
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The Norwegian Radium Hospital, Oslo, Norway. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA
/
genetics,
isolation & purification DNA Mutational Analysis / methods* Electrophoresis, Polyacrylamide Gel / methods* Genetics, Medical Humans Nucleic Acid Denaturation |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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