Document Detail


Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays.
MedLine Citation:
PMID:  22311593     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ankylosing spondylitis (AS) is a common, inflammatory rheumatic disease that primarily affects the axial skeleton and is associated with sacroiliitis, uveitis, and enthesitis. Unlike other autoimmune rheumatic diseases, such as rheumatoid arthritis or systemic lupus erythematosus, autoantibodies have not yet been reported to be a feature of AS. We therefore wished to determine whether plasma from patients with AS contained autoantibodies and, if so, characterize and quantify this response in comparison to patients with rheumatoid arthritis (RA) and healthy controls. Two high density nucleic acid programmable protein arrays expressing a total of 3498 proteins were screened with plasma from 25 patients with AS, 17 with RA, and 25 healthy controls. Autoantigens identified were subjected to Ingenuity Pathway Analysis to determine the patterns of signaling cascades or tissue origin. 44% of patients with ankylosing spondylitis demonstrated a broad autoantibody response, as compared with 33% of patients with RA and only 8% of healthy controls. Individuals with AS demonstrated autoantibody responses to shared autoantigens, and 60% of autoantigens identified in the AS cohort were restricted to that group. The autoantibody responses in the AS patients were targeted toward connective, skeletal, and muscular tissue, unlike those of RA patients or healthy controls. Thus, patients with AS show evidence of systemic humoral autoimmunity and multispecific autoantibody production. Nucleic acid programmable protein arrays constitute a powerful tool to study autoimmune diseases.
Authors:
Cynthia Wright; Sahar Sibani; David Trudgian; Roman Fischer; Benedikt Kessler; Joshua LaBaer; Paul Bowness
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  11     ISSN:  1535-9484     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-07     Completed Date:  2012-06-01     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M9.00384     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Autoantibodies / blood*,  immunology
Autoantigens / immunology*
Case-Control Studies
Female
Humans
Male
Middle Aged
Nucleic Acids / chemistry*
Prognosis
Protein Array Analysis*
Proteome / analysis
Spondylitis, Ankylosing / blood,  diagnosis*,  immunology*
Young Adult
Grant Support
ID/Acronym/Agency:
18599//Arthritis Research UK; //Medical Research Council
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Autoantigens; 0/Nucleic Acids; 0/Proteome
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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