Document Detail

Detection of multiallele polymorphisms within gene sequences by GC-clamped denaturing gradient gel electrophoresis.
MedLine Citation:
PMID:  1539594     Owner:  NLM     Status:  MEDLINE    
The ability to efficiently detect DNA polymorphisms is essential for the completion of a high-resolution polymorphic linkage map of the human genome. Currently the most informative polymorphisms are the multiallelic dinucleotide repeat polymorphisms. However, many gene sequences lack an associated dinucleotide repeat sequence. We used GC-clamped denaturing gradient gel electrophoresis to screen for DNA polymorphisms in the following six gene sequences: MCC, p53, prealbumin (transthyretin), rhodopsin, S-antigen, and TGF-alpha. A single-base sequence polymorphism was identified in each of these gene sequences. Some of these polymorphisms were multiallelic and highly informative. Our results demonstrate the value of denaturing gradient gel electrophoresis for both identifying and analyzing human DNA polymorphisms. The ability to detect highly informative polymorphisms within gene sequences will greatly contribute to a gene-based polymorphic linkage map.
V C Sheffield; J S Beck; B Nichols; A Cousineau; A C Lidral; E M Stone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of human genetics     Volume:  50     ISSN:  0002-9297     ISO Abbreviation:  Am. J. Hum. Genet.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-04-02     Completed Date:  1992-04-02     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0370475     Medline TA:  Am J Hum Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  567-75     Citation Subset:  IM    
Department of Pediatrics University of Iowa, Iowa City.
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MeSH Terms
Antigens / genetics
Base Sequence
Chromosome Mapping / methods*
DNA / analysis
Electrophoresis, Gel, Pulsed-Field
Eye Proteins / genetics
Genes, Tumor Suppressor / genetics
Genes, p53 / genetics
Linkage (Genetics) / genetics*
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic / genetics*
Prealbumin / genetics
Proteins / genetics
Rhodopsin / genetics
Transforming Growth Factor alpha / genetics
Tumor Suppressor Proteins*
Grant Support
Reg. No./Substance:
0/Antigens; 0/Arrestin; 0/Eye Proteins; 0/Prealbumin; 0/Proteins; 0/Transforming Growth Factor alpha; 0/Tumor Suppressor Proteins; 137764-10-4/MCC protein, human; 9007-49-2/DNA; 9009-81-8/Rhodopsin

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