Document Detail


Detection of microcystin-producing cyanobacteria in Finnish lakes with genus-specific microcystin synthetase gene E (mcyE) PCR and associations with environmental factors.
MedLine Citation:
PMID:  16957235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the frequency and composition of potential microcystin (MC) producers in 70 Finnish lakes with general and genus-specific microcystin synthetase gene E (mcyE) PCR. Potential MC-producing Microcystis, Planktothrixand Anabaena spp. existed in 70%, 63%, and 37% of the lake samples, respectively. Approximately two-thirds of the lake samples contained one or two potential MC producers, while all three genera existed in 24% of the samples. In oligotrophic lakes, the occurrence of only one MC producer was most common. The combination of Microcystis and Planktothrix was slightly more prevalent than others in mesotrophic lakes, and the cooccurrence of all three MC producers was most widespread in both eutrophic and hypertrophic lakes. The proportion of the three-producer lakes increased with the trophic status of the lakes. In correlation analysis, the presence of multiple MC-producing genera was associated with higher cyanobacterial and phytoplankton biomass, pH, chlorophyll a, total nitrogen, and MC concentrations. Total nitrogen, pH, and the surface area of the lake predicted the occurrence probability of mcyE genes, whereas total phosphorus alone accounted for MC concentrations in the samples by logistic and linear regression analyses. In conclusion, the results suggested that eutrophication increased the cooccurrence of potentially MC-producing cyanobacterial genera, raising the risk of toxic-bloom formation.
Authors:
Anne Rantala; Pirjo Rajaniemi-Wacklin; Christina Lyra; Liisa Lepistö; Jukka Rintala; Joanna Mankiewicz-Boczek; Kaarina Sivonen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-07     Completed Date:  2006-11-08     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6101-10     Citation Subset:  IM    
Affiliation:
Department of Applied Chemistry and Microbiology, P.O. Box 56 (Viikinkaari 9), FIN-00014 Helsinki University, Finland.
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MeSH Terms
Descriptor/Qualifier:
Anabaena / genetics,  metabolism
Bacterial Proteins / genetics
Base Sequence
Cyanobacteria / genetics*,  isolation & purification,  metabolism*
DNA, Bacterial / genetics
Ecosystem
Finland
Fresh Water / analysis,  microbiology*
Genes, Bacterial*
Linear Models
Logistic Models
Microcystins
Microcystis / genetics,  metabolism
Peptide Synthases / genetics*
Peptides, Cyclic / analysis,  biosynthesis*
Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/Microcystins; 0/Peptides, Cyclic; 77238-39-2/microcystin; EC 6.3.2.-/Peptide Synthases
Comments/Corrections

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