Document Detail

Detection methods of possible prion contaminants in collagen and gelatin.
MedLine Citation:
PMID:  10076518     Owner:  NLM     Status:  MEDLINE    
We describe methods for the preparation of collagen and gelatin samples to detect possible prion contaminants using Western blotting of a major component of prions, PrPSc. A commercially available collagen solution containing 2% athero-collagen was spiked with rodent adapted scrapie prion and used as the prion-contaminating collagen. The methods developed center on the enzymatic reduction of the collagen solution viscosity with protease treatments and on the concentration of the prion from the protease-digests with polyethylene glycol-#6000 and NaCl. Recovery of the spiked prion as a partially protease-resistant core fragment of PrPSc fluctuated from 30% to 46% of the input amount.
T Nemoto; M Horiuchi; N Ishiguro; M Shinagawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of virology     Volume:  144     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  1999  
Date Detail:
Created Date:  1999-03-31     Completed Date:  1999-03-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  AUSTRIA    
Other Details:
Languages:  eng     Pagination:  177-84     Citation Subset:  IM    
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
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MeSH Terms
Blotting, Western / methods
Drug Contamination*
Prions / isolation & purification*
Reg. No./Substance:
0/Prions; 9000-70-8/Gelatin; 9007-34-5/Collagen

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