Document Detail


Detection of metallo-β-lactamase genes in clinical specimens by a commercial multiplex PCR system.
MedLine Citation:
PMID:  20807554     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
hyplex®-MBL ID Multiplex PCR-ELISA, a novel method for identifying metallo-β-lactamase genes directly in clinical specimens, was evaluated using a consecutive collection of 326 samples from three hospitals in Greece characterized by high prevalence of VIM producers. The method exhibited high sensitivity (98.0%) and specificity (98.6%) and was proven reliable in detecting bla(VIM) genes in blood, urine, pus, and sputum samples that, as confirmed by conventional methods, contained various VIM-producing species. Future multicenter studies should be considered for the thorough evaluation of this method and its potential diagnostic utility.
Authors:
Athina Avlami; S Bekris; G Ganteris; Eleni Kraniotaki; Eleni Malamou-Lada; Maria Orfanidou; Olga Paniara; Angeliki Pantazatou; C C Papagiannitsis; Evangelia Platsouka; Ioanna Stefanou; Eva Tzelepi; Helen Vagiakou; Vivi Miriagou
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2010-08-31
Journal Detail:
Title:  Journal of microbiological methods     Volume:  83     ISSN:  1872-8359     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-01-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  185-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Department of Microbiology, Laikon General Hospital, Athens, Greece.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / biosynthesis,  genetics*
Enzyme-Linked Immunosorbent Assay / methods
Gram-Negative Bacteria / enzymology*,  genetics,  isolation & purification
Gram-Negative Bacterial Infections / microbiology*
Greece
Humans
Microbial Sensitivity Tests / methods
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
beta-Lactamases / biosynthesis,  genetics*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; EC 3.5.2.6/beta-Lactamases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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