Document Detail


Detection of mercuric ions in water by ELISA with a mercury-specific antibody.
MedLine Citation:
PMID:  1862940     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An immunoassay that detects mercuric ions in water at concentrations of 0.5 ppb and above is described. The assay utilizes a monoclonal antibody that binds specifically to mercuric ions immobilized in wells of microtiter plates. Within the range of 0.5-10 ppb mercury, the absorbance in the enzyme-linked immunosorbent assay (ELISA) is linear to the log of the mercuric ion concentration. The quantitation of mercury by ELISA correlates closely with results from cold-vapor atomic absorption. Other divalent metal cations do not interfere with the assay, although there is interference in the presence of 1 mM chloride ions. The optimum pH for mercury detection is 7.0, although 2 ppb mercury can be detected over a wide pH range. The assay is as sensitive as cold-vapor atomic absorption for mercury detection and can be performed with only 100 microliters of sample.
Authors:
D E Wylie; L D Carlson; R Carlson; F W Wagner; S M Schuster
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  194     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-08-30     Completed Date:  1991-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-7     Citation Subset:  IM    
Affiliation:
BioNebraska, Inc., Lincoln 68504.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / biosynthesis,  immunology*
Chlorides
Enzyme-Linked Immunosorbent Assay / methods*
Hydrogen-Ion Concentration
Mercury / analysis*,  immunology
Mice
Mice, Inbred BALB C
Spectrophotometry, Atomic
Water / chemistry*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Chlorides; 7439-97-6/Mercury; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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