| Detection of melanoma cells in sentinel lymph nodes by reverse transcriptase-polymerase chain reaction: prognostic significance. | |
| | |
MedLine Citation:
|
PMID: 15525827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Recently reverse transcriptase-polymerase chain reaction (RT-PCR) has been proposed as a new sensitive method for the detection of submicroscopic melanoma nodal metastases. Sentinel lymph node (SLN) status is considered the most important prognostic factor for melanoma patients. Thus, in recent years, melanoma research has been focused on identifying new molecular markers of micrometastases. METHODS: In this study, 129 SLNs were collected and analyzed by RT-PCR for tyrosinase and melanoma inhibitory activity (MIA) messenger RNA (mRNA) expression. RESULTS from PCR analysis were then compared with those obtained by hematoxylin and eosin and immunohistochemistry and related to progression of disease. RESULTS: MIA gene expression was positive by RT-PCR in 27% of the tyrosinase-positive SLNs. When the correlation between tyrosinase and/or MIA mRNA expression and disease-free survival was evaluated by the Kaplan-Meier exact test, there was a statistically significant correlation between simultaneous tyrosinase and MIA gene expression in SLNs and progression of disease. CONCLUSIONS: RT-PCR analysis for both MIA and tyrosinase mRNA may identify a subset of melanoma patients with a worse prognosis whom the routine methods, such as histology and immunohistochemistry, fail to identify because of the poor sensitivity of these methods. |
| | |
Authors:
|
A Gradilone; D Ribuffo; I Silvestri; E Cigna; P Gazzaniga; I Nofroni; G Zamolo; L Frati; N Scuderi; A M Aglianò |
Related Documents
:
|
1916067 - Tissue and age-specificity of post-translational modifications of n-cadherin during chi... 22357957 - Deficiency of porcupine, an o-acyltransferase gene, impairs convergent extension during... 19017487 - Regulation of melanocyte apoptosis by stathmin 1 expression. 17250547 - Inhibition of mitf transcriptional activity independent of targeting p300/cbp coactivat... 7532097 - Neurotrophic factor gene expression in astrocytes during development and following injury. 15501227 - Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal ... |
Publication Detail:
|
Type: Clinical Trial; Journal Article Date: 2004-10-15 |
Journal Detail:
|
Title: Annals of surgical oncology Volume: 11 ISSN: 1068-9265 ISO Abbreviation: Ann. Surg. Oncol. Publication Date: 2004 Nov |
Date Detail:
|
Created Date: 2004-11-04 Completed Date: 2005-02-07 Revised Date: 2007-07-18 |
Medline Journal Info:
|
Nlm Unique ID: 9420840 Medline TA: Ann Surg Oncol Country: United States |
Other Details:
|
Languages: eng Pagination: 983-7 Citation Subset: IM |
Affiliation:
|
Dipartimento di Medicina Sperimentale e Patologia, Viale Regina Elena 324, 00161 Rome, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Disease-Free Survival Extracellular Matrix Proteins Gene Expression Profiling* Humans Melanoma / genetics*, pathology* Monophenol Monooxygenase / biosynthesis, pharmacology Neoplasm Proteins / biosynthesis, pharmacology Prognosis Reverse Transcriptase Polymerase Chain Reaction* Sensitivity and Specificity Sentinel Lymph Node Biopsy* Skin Neoplasms / genetics*, pathology* |
| Chemical | |
Reg. No./Substance:
|
0/Extracellular Matrix Proteins; 0/MIA protein, human; 0/Neoplasm Proteins; EC 1.14.18.1/Monophenol Monooxygenase |
| Comments/Corrections | |
Comment In:
|
Ann Surg Oncol. 2004 Nov;11(11):953-4
[PMID:
15525822
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Microsatellite instability as a prognostic factor in resected colorectal cancer liver metastases.
Next Document: Detection of melanoma cells in the lymphatic drainage after lymph node dissection in melanoma patien...