Document Detail


Detection of melamine using commercial enzyme-linked immunosorbent assay technology.
MedLine Citation:
PMID:  18389705     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent cases of adulteration with melamine have led to the need for rapid and reliable screening methods. To meet this need, commercial enzyme-linked immunosorbent assay (ELISA) test kits for the detection of triazines were evaluated. The recently released Melamine Plate kit (Abraxis, Warminster, Pa.) displayed a limit of detection of 9 ng/ml for melamine in phosphate-buffered saline (PBS) and approximately 1 microg/ml for melamine added to dog food. An atrazine ELISA test kit produced by Abraxis required 0.2 mg/ml to generate a response more than four times the standard deviation from background. In contrast, with the EnviroGard Triazine Plate kit (Strategic Diagnostics, Inc., Newark, Del.), 1.5 mg/ml melamine in PBS generated a signal only one standard deviation from background, which was insufficient to define a limit of detection. Extraction based on dilution with 105 mM sodium phosphate/75 mM NaCl/2.5% nonfat milk/0.05% Tween 20 (UD) enabled detection of fivefold less melamine in dog food than did use of the procedure recommended by the manufacturer, which entailed extraction into 60% methanol, sonication, centrifugation, filtration, and further dilution into 10% methanol/PBS. Using the Abraxis Melamine ELISA, both extraction protocols yielded identical results with a dog food sample adulterated with melamine. The recovery of melamine spiked into gravy from dog food using UD was 74% +/- 4%. In conclusion, the recently released Abraxis ELISA for melamine proved to be a useful alternative to more cumbersome methods.
Authors:
Eric A E Garber
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  71     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-04-07     Completed Date:  2008-05-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  590-4     Citation Subset:  IM    
Affiliation:
Division of Bioanalytical Chemistry, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, Maryland 20740, USA. eric.garber@fda.hhs.gov
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MeSH Terms
Descriptor/Qualifier:
Animal Feed / analysis*
Animals
Consumer Product Safety
Enzyme-Linked Immunosorbent Assay / methods*
Food Contamination / analysis*
Herbicides / analysis*
Humans
Pesticide Residues / analysis
Reagent Kits, Diagnostic
Reproducibility of Results
Sensitivity and Specificity
Triazines / analysis*
Chemical
Reg. No./Substance:
0/Herbicides; 0/Pesticide Residues; 0/Reagent Kits, Diagnostic; 0/Triazines; 108-78-1/melamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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