| Detection of melamine using commercial enzyme-linked immunosorbent assay technology. | |
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MedLine Citation:
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PMID: 18389705 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent cases of adulteration with melamine have led to the need for rapid and reliable screening methods. To meet this need, commercial enzyme-linked immunosorbent assay (ELISA) test kits for the detection of triazines were evaluated. The recently released Melamine Plate kit (Abraxis, Warminster, Pa.) displayed a limit of detection of 9 ng/ml for melamine in phosphate-buffered saline (PBS) and approximately 1 microg/ml for melamine added to dog food. An atrazine ELISA test kit produced by Abraxis required 0.2 mg/ml to generate a response more than four times the standard deviation from background. In contrast, with the EnviroGard Triazine Plate kit (Strategic Diagnostics, Inc., Newark, Del.), 1.5 mg/ml melamine in PBS generated a signal only one standard deviation from background, which was insufficient to define a limit of detection. Extraction based on dilution with 105 mM sodium phosphate/75 mM NaCl/2.5% nonfat milk/0.05% Tween 20 (UD) enabled detection of fivefold less melamine in dog food than did use of the procedure recommended by the manufacturer, which entailed extraction into 60% methanol, sonication, centrifugation, filtration, and further dilution into 10% methanol/PBS. Using the Abraxis Melamine ELISA, both extraction protocols yielded identical results with a dog food sample adulterated with melamine. The recovery of melamine spiked into gravy from dog food using UD was 74% +/- 4%. In conclusion, the recently released Abraxis ELISA for melamine proved to be a useful alternative to more cumbersome methods. |
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Authors:
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Eric A E Garber |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of food protection Volume: 71 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-04-07 Completed Date: 2008-05-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
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Languages: eng Pagination: 590-4 Citation Subset: IM |
Affiliation:
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Division of Bioanalytical Chemistry, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, Maryland 20740, USA. eric.garber@fda.hhs.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed
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analysis* Animals Consumer Product Safety Enzyme-Linked Immunosorbent Assay / methods* Food Contamination / analysis* Herbicides / analysis* Humans Pesticide Residues / analysis Reagent Kits, Diagnostic Reproducibility of Results Sensitivity and Specificity Triazines / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Herbicides; 0/Pesticide Residues; 0/Reagent Kits, Diagnostic; 0/Triazines; 108-78-1/melamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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