| Detection and manipulation of mitochondrial reactive oxygen species in mammalian cells. | |
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MedLine Citation:
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PMID: 20100455 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reactive oxygen species (ROS) are formed upon incomplete reduction of molecular oxygen (O2) as an inevitable consequence of mitochondrial metabolism. Because ROS can damage biomolecules, cells contain elaborate antioxidant defense systems to prevent oxidative stress. In addition to their damaging effect, ROS can also operate as intracellular signaling molecules. Given the fact that mitochondrial ROS appear to be only generated at specific sites and that particular ROS species display a unique chemistry and have specific molecular targets, mitochondria-derived ROS might constitute local regulatory signals. The latter would allow individual mitochondria to auto-regulate their metabolism, shape and motility, enabling them to respond autonomously to the metabolic requirements of the cell. In this review we first summarize how mitochondrial ROS can be generated and removed in the living cell. Then we discuss experimental strategies for (local) detection of ROS by combining chemical or proteinaceous reporter molecules with quantitative live cell microscopy. Finally, approaches involving targeted pro- and antioxidants are presented, which allow the local manipulation of ROS levels. |
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Authors:
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Marleen Forkink; Jan A M Smeitink; Roland Brock; Peter H G M Willems; Werner J H Koopman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2010-01-25 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1797 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2010 Jun-Jul |
Date Detail:
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Created Date: 2010-06-21 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1034-44 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / metabolism Electron Transport Complex I / metabolism Electron Transport Complex III / metabolism Fluorescent Dyes Humans Ketoglutarate Dehydrogenase Complex / metabolism Luminescent Proteins / metabolism Mammals / metabolism Mitochondria / metabolism* Oxidants / metabolism Reactive Oxygen Species / metabolism* Signal Transduction |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Fluorescent Dyes; 0/Luminescent Proteins; 0/Oxidants; 0/Reactive Oxygen Species; EC 1.10.2.2/Electron Transport Complex III; EC 1.2.4.2/Ketoglutarate Dehydrogenase Complex; EC 1.6.5.3/Electron Transport Complex I |
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