| Detection of malondialdehyde in vivo using microdialysis sampling with CE-fluorescence. | |
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MedLine Citation:
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PMID: 22034011 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidative damage is a naturally occurring process where reactive oxygen species (ROS) attack and disrupt normal cellular function; however, these effects become elevated during a stress event, such as ischemia/reperfusion or seizure. One result of oxidative stress is lipid peroxidation, where ROS attack free unsaturated fatty acids forming lipid hydorperoxides, which then break down to form secondary products acrolein, 4-hydroxynonenal, and malondialdehyde (MDA) resulting in irreversible membrane damage and ultimately cell death. Described here is a CE-fluorescence method for the determination of MDA in conjunction with in vivo microdialysis sampling. MDA was derivatized with thiobarbituric acid under acidic conditions for 20 minutes and injected directly into the capillary without any pretreatment. This method provided a limit of detection of 25 nM (S/N=3) and a linear range of 25-2400 nM (1.8-174 ng/mL). This method was used to quantify MDA in rat heart, muscle, liver, and brain dialysate. |
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Authors:
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Justin Carl Cooley; Craig Edward Lunte |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Electrophoresis Volume: 32 ISSN: 1522-2683 ISO Abbreviation: Electrophoresis Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-10-28 Completed Date: 2012-01-12 Revised Date: 2013-04-01 |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 2994-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Department of Chemistry, University of Kansas, Lawrence, KS 66047, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Chemistry Electrophoresis, Capillary / methods* Hydrogen-Ion Concentration Linear Models Liver / chemistry Malondialdehyde / analysis* Microdialysis / methods* Muscles / chemistry Myocardium / chemistry Oxidative Stress Rats Rats, Sprague-Dawley Sensitivity and Specificity Thiobarbiturates |
| Grant Support | |
ID/Acronym/Agency:
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R01 NS066466/NS/NINDS NIH HHS; R01-NS066466/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Thiobarbiturates; 542-78-9/Malondialdehyde; M1YZW5SS7C/thiobarbituric acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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