Document Detail


Detection of malondialdehyde in vivo using microdialysis sampling with CE-fluorescence.
MedLine Citation:
PMID:  22034011     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oxidative damage is a naturally occurring process where reactive oxygen species (ROS) attack and disrupt normal cellular function; however, these effects become elevated during a stress event, such as ischemia/reperfusion or seizure. One result of oxidative stress is lipid peroxidation, where ROS attack free unsaturated fatty acids forming lipid hydorperoxides, which then break down to form secondary products acrolein, 4-hydroxynonenal, and malondialdehyde (MDA) resulting in irreversible membrane damage and ultimately cell death. Described here is a CE-fluorescence method for the determination of MDA in conjunction with in vivo microdialysis sampling. MDA was derivatized with thiobarbituric acid under acidic conditions for 20 minutes and injected directly into the capillary without any pretreatment. This method provided a limit of detection of 25 nM (S/N=3) and a linear range of 25-2400 nM (1.8-174 ng/mL). This method was used to quantify MDA in rat heart, muscle, liver, and brain dialysate.
Authors:
Justin Carl Cooley; Craig Edward Lunte
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Electrophoresis     Volume:  32     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-28     Completed Date:  2012-01-12     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2994-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Department of Chemistry, University of Kansas, Lawrence, KS 66047, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Chemistry
Electrophoresis, Capillary / methods*
Hydrogen-Ion Concentration
Linear Models
Liver / chemistry
Malondialdehyde / analysis*
Microdialysis / methods*
Muscles / chemistry
Myocardium / chemistry
Oxidative Stress
Rats
Rats, Sprague-Dawley
Sensitivity and Specificity
Thiobarbiturates
Grant Support
ID/Acronym/Agency:
R01 NS066466/NS/NINDS NIH HHS; R01-NS066466/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Thiobarbiturates; 542-78-9/Malondialdehyde; M1YZW5SS7C/thiobarbituric acid
Comments/Corrections

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