Document Detail

Detection of lipopolysaccharides in polyacrylamide gels by transfer to nitrocellulose followed by immunoautoradiography with antibody and 125I-protein A: "LPS blotting".
MedLine Citation:
PMID:  6203428     Owner:  NLM     Status:  MEDLINE    
Lipopolysaccharides (LPS), which constitute the somatic (O) antigen of gram-negative bacteria, were used to demonstrate the procedure of LPS blotting involving the electrophoretic transfer of electrophoretically resolved LPS from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose filters. Immobilized LPS could then be immunoautoradiographically visualized in situ by reaction with specific anti-LPS antibody and subsequent binding of radioiodinated Staphylococcus protein A. LPS blotting is expected to provide an efficient and specific means of investigating the LPS (O) antigens of gram-negative bacteria.
W C Bradbury; S D Mills; M A Preston; L J Barton; J L Penner
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical biochemistry     Volume:  137     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1984 Feb 
Date Detail:
Created Date:  1984-07-02     Completed Date:  1984-07-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  129-33     Citation Subset:  IM    
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MeSH Terms
Antibodies, Bacterial / immunology
Antibody Specificity
Electrophoresis, Polyacrylamide Gel / methods*
Iodine Radioisotopes
Lipopolysaccharides / analysis*,  immunology,  isolation & purification
Staining and Labeling
Staphylococcal Protein A / immunology
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Iodine Radioisotopes; 0/Lipopolysaccharides; 0/Staphylococcal Protein A; 7440-22-4/Silver; 9004-70-0/Collodion

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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