| Detection of lipopolysaccharides in polyacrylamide gels by transfer to nitrocellulose followed by immunoautoradiography with antibody and 125I-protein A: "LPS blotting". | |
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MedLine Citation:
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PMID: 6203428 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipopolysaccharides (LPS), which constitute the somatic (O) antigen of gram-negative bacteria, were used to demonstrate the procedure of LPS blotting involving the electrophoretic transfer of electrophoretically resolved LPS from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose filters. Immobilized LPS could then be immunoautoradiographically visualized in situ by reaction with specific anti-LPS antibody and subsequent binding of radioiodinated Staphylococcus protein A. LPS blotting is expected to provide an efficient and specific means of investigating the LPS (O) antigens of gram-negative bacteria. |
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Authors:
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W C Bradbury; S D Mills; M A Preston; L J Barton; J L Penner |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 137 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1984 Feb |
Date Detail:
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Created Date: 1984-07-02 Completed Date: 1984-07-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 129-33 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Bacterial
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immunology Antibody Specificity Autoradiography Collodion* Electrophoresis, Polyacrylamide Gel / methods* Iodine Radioisotopes Lipopolysaccharides / analysis*, immunology, isolation & purification Radioimmunoassay* Silver Staining and Labeling Staphylococcal Protein A / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bacterial; 0/Iodine Radioisotopes; 0/Lipopolysaccharides; 0/Staphylococcal Protein A; 7440-22-4/Silver; 9004-70-0/Collodion |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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