Document Detail


Detection of lipopolysaccharides by ethidium bromide staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
MedLine Citation:
PMID:  1688839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and easy method for staining lipopolysaccharides with ethidium bromide is described. Lipopolysaccharides could be visualized by ethidium bromide with almost the same sensitivity as found with the silver-staining method in less than 30 min. The ethidium bromide-staining method was particularly suitable for staining lipopolysaccharides possessing acidic O-specific polysaccharides, which were poorly visualized by silver staining.
Authors:
N Kido; M Ohta; N Kato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  172     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-03-14     Completed Date:  1990-03-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1145-7     Citation Subset:  IM    
Affiliation:
Department of Bacteriology, Nagoya University School of Medicine, Aichi, Japan.
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MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Polyacrylamide Gel / methods
Escherichia coli / immunology
Ethidium
Lipopolysaccharides / analysis*,  isolation & purification
Salmonella typhimurium / immunology
Shigella / immunology
Staining and Labeling
Chemical
Reg. No./Substance:
0/Lipopolysaccharides; 3546-21-2/Ethidium
Comments/Corrections

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