Document Detail


Detection of lecithin: cholesterol acyltransferase (LCAT) in a human hepatoma cell line.
MedLine Citation:
PMID:  2983879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human hepatoma cell line (HepG-2) was probed for the presence of lecithin: cholesterol acyltransferase (LCAT) using an antiserum to human plasma LCAT. Double immunodiffusion analysis using antiserum to human plasma LCAT revealed a single precipitin line in the sonicated cell homogenate. This precipitin line showed a reaction of identity with highly purified plasma LCAT. The presence of LCAT within the hepatoma cells was also confirmed by an immunofluorescence test. In contrast, the cell culture supernate showed a weak and inconsistent precipitin line. These data suggest that HepG-2 cells synthesize LCAT but secretion of the enzyme by these cells into the culture medium may be partially or totally impaired.
Authors:
E Koren; W J McConathy; A G Lacko; B Knowles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer biochemistry biophysics     Volume:  7     ISSN:  0305-7232     ISO Abbreviation:  Cancer Biochem. Biophys.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-05-14     Completed Date:  1985-05-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7506524     Medline TA:  Cancer Biochem Biophys     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  301-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Hepatocellular / enzymology*
Cell Line
Fluorescent Antibody Technique
Humans
Immunodiffusion
Immunoelectrophoresis
Liver Neoplasms / enzymology*
Phosphatidylcholine-Sterol O-Acyltransferase / analysis*
Grant Support
ID/Acronym/Agency:
AG-03255/AG/NIA NIH HHS; HL-23181/HL/NHLBI NIH HHS; HL-23719/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
EC 2.3.1.43/Phosphatidylcholine-Sterol O-Acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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