Document Detail


Detection, isolation, and characterization of acidophilic methanotrophs from Sphagnum mosses.
MedLine Citation:
PMID:  21724892     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sphagnum peatlands are important ecosystems in the methane cycle. Methane-oxidizing bacteria in these ecosystems serve as a methane filter and limit methane emissions. Yet little is known about the diversity and identity of the methanotrophs present in and on Sphagnum mosses of peatlands, and only a few isolates are known. The methanotrophic community in Sphagnum mosses, originating from a Dutch peat bog, was investigated using a pmoA microarray. A high biodiversity of both gamma- and alphaproteobacterial methanotrophs was found. With Sphagnum mosses as the inoculum, alpha- and gammaproteobacterial acidophilic methanotrophs were isolated using established and newly designed media. The 16S rRNA, pmoA, pxmA, and mmoX gene sequences showed that the alphaproteobacterial isolates belonged to the Methylocystis and Methylosinus genera. The Methylosinus species isolated are the first acid-tolerant members of this genus. Of the acidophilic gammaproteobacterial strains isolated, strain M5 was affiliated with the Methylomonas genus, and the other strain, M200, may represent a novel genus, most closely related to the genera Methylosoma and Methylovulum. So far, no acidophilic or acid-tolerant methanotrophs in the Gammaproteobacteria class are known. All strains showed the typical features of either type I or II methanotrophs and are, to the best of our knowledge, the first isolated (acidophilic or acid-tolerant) methanotrophs from Sphagnum mosses.
Authors:
Nardy Kip; Wenjing Ouyang; Julia van Winden; Ashna Raghoebarsing; Laura van Niftrik; Arjan Pol; Yao Pan; Levente Bodrossy; Elly G van Donselaar; Gert-Jan Reichart; Mike S M Jetten; Jaap S Sinninghe Damsté; Huub J M Op den Camp
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-08-05     Completed Date:  2011-12-15     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5643-54     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Nijmegen, The Netherlands.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ076754;  DQ076755;  DQ076756;  HM564015;  HM564016;  HM564017;  HM564018;  HM564019;  HM564020;  HM564021;  JN036511;  JN036512;  JN036513;  JN036514;  JN036515;  JN036516;  JN036517;  JN036518;  JN036519;  JN036520;  JN036521;  JN036522;  JN036523;  JN036524;  JN036525;  JN036526;  JN036527;  JN036528
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MeSH Terms
Descriptor/Qualifier:
Acids / metabolism
Bacterial Typing Techniques
Base Sequence
Culture Media / chemistry
DNA, Bacterial / genetics
Ecosystem
Genes, Bacterial
Methane / metabolism
Microscopy, Electron, Transmission
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis*
Oxidation-Reduction
Phospholipids / metabolism
Proteobacteria / classification,  genetics,  isolation & purification*,  ultrastructure
RNA, Ribosomal, 16S / genetics
Soil Microbiology*
Sphagnopsida / microbiology*
Chemical
Reg. No./Substance:
0/Acids; 0/Culture Media; 0/DNA, Bacterial; 0/Phospholipids; 0/RNA, Ribosomal, 16S; 74-82-8/Methane
Comments/Corrections

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