| Detection of the inhibitory neurotransmitter GABA in macrophages by magnetic resonance spectroscopy. | |
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MedLine Citation:
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PMID: 15908457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Macrophages are key components of the inflammatory response to tissue injury, but their activities can exacerbate neuropathology. High-resolution magnetic resonance spectroscopy was used to identify metabolite levels in perchloric acid extracts of cultured cells of the RAW 264.7 murine macrophage line under resting and lipopolysaccharide-activated conditions. Over 25 metabolites were identified including gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter not previously reported to be present in macrophages. The presence of GABA was also demonstrated in extracts of human peripheral blood monocyte-derived macrophages. This finding suggests that there may be communication between damaged central nervous system (CNS) tissue and recruited macrophages and resident microglia, which could help orchestrate the immune response. On activation, lactate, glutamine, glutamate, and taurine levels were elevated significantly, and GABA and alanine were reduced significantly. Strong resonances from glutathione, evident in the macrophage two-dimensional 1H spectrum, suggest that this may have potential as a noninvasive marker of macrophages recruited to the CNS, as it is only present at low levels in normal brain. Alternatively, a specific combination of spectroscopic changes, such as lactate, alanine, glutathione, and polyamines, may prove to be the most accurate means of detecting macrophage recruitment to the CNS. |
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Authors:
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D J Stuckey; D C Anthony; J P Lowe; J Miller; W M Palm; P Styles; V H Perry; A M Blamire; N R Sibson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-05-20 |
Journal Detail:
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Title: Journal of leukocyte biology Volume: 78 ISSN: 0741-5400 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-07-29 Completed Date: 2005-10-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 393-400 Citation Subset: IM |
Affiliation:
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Experimental Neuroimaging Group, Department of Biochemistry, University of Oxford, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Biological Markers / analysis Brain Injuries / immunology, metabolism Cell Communication / immunology Cell Extracts / agonists* Cell Line Cell Movement / immunology Humans Inflammation / immunology, metabolism Lactic Acid / analysis Macrophage Activation* / immunology Macrophages / chemistry*, immunology, metabolism Magnetic Resonance Spectroscopy* Mice Microglia / immunology, metabolism Taurine / analysis gamma-Aminobutyric Acid / analysis*, immunology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Biological Markers; 0/Cell Extracts; 107-35-7/Taurine; 50-21-5/Lactic Acid; 56-12-2/gamma-Aminobutyric Acid |
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