Document Detail


Detection of increased choline compounds with proton nuclear magnetic resonance spectroscopy subsequent to malignant transformation of human prostatic epithelial cells.
MedLine Citation:
PMID:  11325827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, a panel of normal human prostate cells (HPCs) and tumor cells derived from metastases were studied by (1)H NMR spectroscopy to determine whether the malignant transformation of HPCs results in the elevation of choline compounds. Although an elevated choline signal has been observed previously in clinical studies, the contribution of the different Cho compounds to this elevation, as well as their quantification, has not been established until now. Here we have shown that HPCs derived from metastases exhibit significantly higher phosphocholine as well as glycerophosphocholine levels compared with normal prostate epithelial and stromal cells. Thus the elevation of the choline peak observed clinically in prostate cancer is attributable to an alteration of phospholipid metabolism and not simply to increased cell density, doubling time, or other nonspecific effects. Androgen deprivation of the androgen receptor-positive cell lines resulted in a significant increase of choline compounds after chronic androgen deprivation of the LNCaP cell line and in a decrease of choline compounds after a more acute androgen deprivation of the LAPC-4 cell line. These data strongly support the use of proton magnetic resonance spectroscopic imaging to detect the presence of prostate cancer for diagnosis, to detect response subsequent to androgen ablation therapy, and to detect recurrence.
Authors:
E Ackerstaff; B R Pflug; J B Nelson; Z M Bhujwalla
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  61     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-04-30     Completed Date:  2001-05-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3599-603     Citation Subset:  IM    
Affiliation:
Magnetic Resonance Oncology Section, Division of Magnetic Resonance Research, Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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MeSH Terms
Descriptor/Qualifier:
Androgens / deficiency,  physiology
Cell Line
Cell Transformation, Neoplastic / metabolism*
Choline / metabolism*
Epithelial Cells / metabolism,  pathology
Humans
Male
Neoplasm Metastasis
Neoplasms, Hormone-Dependent / metabolism
Nuclear Magnetic Resonance, Biomolecular
Phosphatidylcholines / metabolism
Phosphorylcholine / metabolism
Prostate / metabolism*,  pathology
Prostatic Neoplasms / metabolism*,  pathology
Receptors, Androgen / metabolism,  physiology
Grant Support
ID/Acronym/Agency:
1RO1 CA73850/CA/NCI NIH HHS; CA74090-03/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Androgens; 0/Phosphatidylcholines; 0/Receptors, Androgen; 107-73-3/Phosphorylcholine; 62-49-7/Choline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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