| Detection of the in vivo conversion of 2-pyrrolidinone to gamma-aminobutyric acid in mouse brain. | |
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MedLine Citation:
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PMID: 427258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Labeled gamma-aminobutyric acid was detected in mouse brain following intravenous injections of deuterium labeled 2-pyrrolidinone. [2H6]Pyrrolidinone was prepared by the reduction of [2H4]succinimide with lithium aluminum deuteride. Quantification was accomplished by a gas chromatography mass spectrometry assay method. gamma-Aminobutyric acid and internal standard, 5-aminovaleric acid, were converted to volatile derivatives by treatment with N,N-dimethylformamide dimethyl acetal. Quantitative estimates were derived from peak area measurements obtained from monitoring the parent ions of the gamma-aminobutyric acid and internal standard derivatives by repetitive scanning during the GC run. The conversion of pyrrolidinone to gamma-aminobutyric acid may provide a method for labeling central gamma-aminobutyric acid pools. |
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Authors:
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P S Callery; M Stogniew; L A Geelhaar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biomedical mass spectrometry Volume: 6 ISSN: 0306-042X ISO Abbreviation: Biomed. Mass Spectrom. Publication Date: 1979 Jan |
Date Detail:
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Created Date: 1979-06-26 Completed Date: 1979-06-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0430246 Medline TA: Biomed Mass Spectrom Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 23-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism Brain Chemistry* Chromatography, Gas Deuterium Isotope Labeling Male Mass Spectrometry / methods* Mice Pyrrolidinones / chemical synthesis, metabolism*, pharmacology gamma-Aminobutyric Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Pyrrolidinones; 56-12-2/gamma-Aminobutyric Acid; 7782-39-0/Deuterium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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