Document Detail

Detection of t(12;21) in childhood acute lymphoblastic leukemia by fluorescence in situ hybridization.
MedLine Citation:
PMID:  10198615     Owner:  NLM     Status:  MEDLINE    
Metaphase preparations from 36 patients with acute lymphoblastic leukemia (ALL) have been retrospectively screened by fluorescence in situ hybridization (FISH) to determine the incidence of translocation (12;21) and the potential usefulness of FISH as an adjunct to conventional cytogenetic analysis. With the use of specific chromosome paints, 4 of 31 patients with B-lineage childhood ALL (13%) demonstrated rearrangements of chromosomes 12 and 21, and therefore, were considered to harbor the translocation, which had not previously been detected by conventional karyotyping. However, none of these positive cases revealed the standard reciprocal t(12;21)(p12;q22) as the sole abnormality involving chromosomes 12 and 21. The study confirms the feasibility and advantages of introducing FISH screening for t(12;21) in pediatric ALL cases and demonstrates the usefulness of FISH screening as a backup to concurrent cytogenetic analysis to resolve variant translocations and aberrant results. The presence of t(12;21) has also been correlated to clinical data to assess the prognostic significance of this translocation on its own or in association with other prognostic features.
D H Spathas; J Stewart; I O Singer; A Theriault; M Bovey; J M Connor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  110     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-04-21     Completed Date:  1999-04-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-13     Citation Subset:  IM    
Duncan Guthrie Institute of Medical Genetics, University of Glasgow, United Kingdom.
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MeSH Terms
Child, Preschool
Chromosomes, Human, Pair 12*
Chromosomes, Human, Pair 21*
Core Binding Factor Alpha 2 Subunit
DNA-Binding Proteins*
In Situ Hybridization, Fluorescence*
Middle Aged
Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
Proto-Oncogene Proteins*
Transcription Factors / genetics
Translocation, Genetic*
Reg. No./Substance:
0/Core Binding Factor Alpha 2 Subunit; 0/DNA-Binding Proteins; 0/Proto-Oncogene Proteins; 0/RUNX1 protein, human; 0/Transcription Factors

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