Document Detail

Detection in BHK cells of a precursor form for lamin A.
MedLine Citation:
PMID:  4065220     Owner:  NLM     Status:  MEDLINE    
Lamins are structural proteins found in the fibrous lamina underlining the nuclear envelope. In vitro translation of polyadenylated RNA or polysomes followed by immunoprecipitation with a serum raised against BHK nuclear matrix proteins showed that lamin A (72 kD) is synthesized as a high molecular weight precursor (74 kD) (Laliberté et al., J Cell Biol 98 (1984) 980) [23]. We have thus investigated the presence in BHK cells of this putative precursor by in vivo labelling with [35S]methionine and immunoprecipitation of lamin proteins. Short labelling times, ranging from 5 to 60 min reveal the presence of the 74 kD protein. Pulse-chase experiments indicate that the half-life of the precursor is about 60 min. On two-dimensional gel, the 74 kD protein is resolved in a cluster of isovariants between pH 7.4 and 6.6, which are generally slightly more alkaline than their counterparts in lamin A. These results indicate that lamin A is synthesized as a precursor of 74 kD; the long half-life further suggests that pre-lamin A might accumulate in some sort of cellular pool before undergoing post-transcriptional modification(s) to give the mature form of lamin A.
A Dagenais; V Bibor-Hardy; J F Laliberté; A Royal; R Simard
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  161     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-01-06     Completed Date:  1986-01-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  269-76     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Nucleus / analysis
Fibroblasts / analysis*
Lamin Type A
Nucleoproteins / analysis*,  biosynthesis
Protein Precursors / analysis*
Protein Processing, Post-Translational
Reg. No./Substance:
0/Lamin Type A; 0/Lamins; 0/Nucleoproteins; 0/Protein Precursors

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