| Detection of immunoregulatory lipid-like factors in human amniotic fluid. | |
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MedLine Citation:
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PMID: 2288647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The immunosuppressive activity of amniotic fluid (AF) is extensively documented in the mouse. Although this property is due in part to the presence of alpha-fetoprotein (alpha-FP), other immunosuppressive factors are suspected. In this article, we demonstrate that human amniotic fluid lipid extract (AFLE) is inhibitory of, although not cytotoxic to, PHA-activated human lymphocytes, of mouse bone marrow cells, and of different established cell lines of human and mouse source. This effect is shown to be reversible. Under preparative thin layer chromatography (TLC) using chloroform:methanol:water (60:38:8) as solvents, the activity of AFLE migrates to two peaks of inhibition with Rf values of 0.46-0.62 and 0.84-1, respectively. These lipid-like factors may play a role as a nonspecific immunoregulatory mechanism which prevents maternally mediated immune rejection of the conceptus. |
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Authors:
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R Rueda; M L Vargas; M García-Pacheco; E García-Olivares |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 24 ISSN: 1046-7408 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 1990 Oct |
Date Detail:
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Created Date: 1991-04-03 Completed Date: 1991-04-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: DENMARK |
Other Details:
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Languages: eng Pagination: 40-4 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Hospital Universitario, Universidad de Granada, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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immunology* Animals Cell Line Chromatography, Thin Layer Humans Leukocytes, Mononuclear / metabolism Lipids / analysis, physiology* Lymphocyte Activation / physiology Mice Mice, Inbred BALB C Suppressor Factors, Immunologic / analysis*, chemistry Thymidine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Lipids; 0/Suppressor Factors, Immunologic; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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