| Detection of immunoglobulin gene rearrangement in B lymphoid malignancies by polymerase chain reaction gene amplification. | |
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MedLine Citation:
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PMID: 2334634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunoglobulin heavy chain gene rearrangement serves as a marker of cell lineage and clonality in B lymphoproliferative disorders. We have used polymerase chain reaction (PCR) gene amplification to detect immunoglobulin gene rearrangements involving VH251, a heavy chain variable region preferentially utilized in B lymphoproliferative disorders. Using synthetic amplimers derived from VH251 and the heavy chain joining region, under conditions of high stringency, a homogeneous VH251-specific fragment of approximately 350 bp could be amplified from leukaemic DNA. Of 53 cases of B lineage acute lymphoblastic leukaemia screened for VH251 rearrangement by PCR, 10 were positive. A background level of VH251 rearrangement could also be amplified from normal peripheral blood and bone marrow DNA, but a VH251 rearranged leukaemic clone representing 0.01% of bone marrow mononuclear cells could be readily detected. The application of PCR to detect immunoglobulin gene rearrangement involving VH251 and potentially other preferentially utilized V regions provides a sensitive method both for tracking malignant B cells and for the study of normal B cell developmental pathways through which B lineage malignancies arise. |
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Authors:
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M Deane; J D Norton |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of haematology Volume: 74 ISSN: 0007-1048 ISO Abbreviation: Br. J. Haematol. Publication Date: 1990 Mar |
Date Detail:
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Created Date: 1990-06-08 Completed Date: 1990-06-08 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372544 Medline TA: Br J Haematol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 251-6 Citation Subset: IM |
Affiliation:
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Department of Haematology, Royal Free Hospital School of Medicine, London. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Southern Bone Marrow Cells Burkitt Lymphoma / genetics* DNA, Neoplasm / genetics Gene Rearrangement, B-Lymphocyte, Heavy Chain* Humans Immunoglobulin Variable Region / genetics Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Immunoglobulin Variable Region |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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