| Detection and identification of trypanosomes by polymerase chain reaction in wild tsetse flies in Cameroon. | |
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MedLine Citation:
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PMID: 9707369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The prevalence of various species and subgroups of trypanosomes in infected flies from three sleeping sickness foci in Cameroon was determined by the use of polymerase chain reaction (PCR). The predominant tsetse species found were Glossina palpalis palpalis. Microscopical examination of 943 non-teneral tsetse flies revealed an average infection rate of 10.4%. A total of 90 flies were analyzed for trypanosome identification with primer sets specific for Trypanosoma (Trypanozoon) brucei s.l., T. (Duttonella) vitax, T. (Nannomonas) simiae, and forest type T. (Nannomonas) congolense. PCR succeeded in identifying 52 of the 90 infected flies. Other primers were also tested on microscope positive/PCR-negative infections, and trypanosome subgroups were detected (Kilifi type and savannah type T. congolense). PCR amplification allowed identification of immature infections and revealed mixed-infections. The PCR technique failed to identify 42.2% (38/90) of the parasitologically positive flies and the reasons for this failure are discussed. |
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Authors:
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I Morlais; P Grebaut; J M Bodo; S Djoha; G Cuny; S Herder |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta tropica Volume: 70 ISSN: 0001-706X ISO Abbreviation: Acta Trop. Publication Date: 1998 Jun |
Date Detail:
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Created Date: 1998-10-06 Completed Date: 1998-10-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370374 Medline TA: Acta Trop Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 109-17 Citation Subset: IM |
Affiliation:
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Laboratoire d'Epidémiologie des Maladies à Vecteurs, Centre ORSTOM, Montpellier, France. cuny@orstom.rio.net |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cameroon Female Insect Vectors / parasitology* Male Polymerase Chain Reaction* Predictive Value of Tests Trypanosoma / isolation & purification* Trypanosoma brucei brucei / isolation & purification Trypanosoma congolense / isolation & purification Trypanosoma vivax / isolation & purification Tsetse Flies / parasitology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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