Document Detail


Detection and identification of the H5 hemagglutinin subtype by real-time RT-PCR.
MedLine Citation:
PMID:  18370038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Serological methods, gene sequencing, and RT-PCR-based methods have all been used for the identification of influenza virus hemagglutinin (HA) subtypes. Compared to serological methods and gene sequencing, RT-PCR is fast, sensitive, and relatively inexpensive. However, since RT-PCR generally lacks the specificity of sequencing or serology, the most practical application of RT-PCR methods for subtype identification is either to target a few of the most important subtypes such as H5 and H7 or to use it in situations where a specific strain is being targeted, such as during an outbreak or with experimental samples. Since identification of viruses from the Asian-origin H5N1 highly pathogenic avian influenza virus lineage is a high priority worldwide, the procedure for real-time RT-PCR (rRT-PCR) identification of the H5 H subtype is presented here. This assay can identify the H5 hemagglutinin of any genetic lineage (North American or Asian) and either pathotype (highly pathogenic or low pathogenic), but does not differentiate between subtypes or pathotypes.
Authors:
Erica Spackman; David L Suarez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  436     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-03-28     Completed Date:  2008-07-02     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  IM    
Affiliation:
Southeast Poultry Research Laboratory, US Department of Agriculture, Agricultural Research Service, Athens, GA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Birds / virology
Hemagglutinin Glycoproteins, Influenza Virus / immunology*
Influenza A Virus, H5N1 Subtype / genetics*,  immunology
Reverse Transcriptase Polymerase Chain Reaction / methods*
Chemical
Reg. No./Substance:
0/Hemagglutinin Glycoproteins, Influenza Virus; 0/hemagglutinin, avian influenza A virus

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