Document Detail

Detection of hydroxyl radical in intact cells of Chlorella vulgaris.
MedLine Citation:
PMID:  7647919     Owner:  NLM     Status:  MEDLINE    
Using ESR with 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin-trapping reagent, we measured the levels of free radical species generated from living cells of Chlorella vulgaris var. vulgails (IAM C-534). To investigate the production of free radicals in the living Chlorella vulgaris cells, the influence of DMPO toward the intact cells of the Chlorella vulgaris using the O2 evolution rate was first studied as a guide. Since the O2 evolution rate was not changed by DMPO, it was judged that DMPO has no toxicity toward the intact cells of Chlorella vulgaris. Only hydroxyl radicals (.OH) were detected as the DMPO-OH adduct in the suspension of intact cells of Chlorella vulgaris irradiated with visible light. Moreover, since production of .OH was inhibited by some hydroxyl radical scavengers such as KI and ethanol, production of .OH was proved to be due to hydroxyl radicals. It was also clear that the intensity of .OH increased with increasing irradiation intensity of visible light. Therefore, it was suggested that .OH might be one of the photoinhibition factors of the intact Chlorella vulgaris cells in severe light conditions.
S Hirayama; R Ueda; K Sugata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Free radical research     Volume:  23     ISSN:  1071-5762     ISO Abbreviation:  Free Radic. Res.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-09-26     Completed Date:  1995-09-26     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  51-9     Citation Subset:  IM    
Bio Technology Research Laboratory, Mitsubishi Heavy Industries, Ltd., Yokohama, Japan.
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MeSH Terms
Chlorella / chemistry*
Cyclic N-Oxides
Electron Spin Resonance Spectroscopy
Free Radicals
Hydroxyl Radical*
Reactive Oxygen Species
Reg. No./Substance:
0/Cyclic N-Oxides; 0/Free Radicals; 0/Reactive Oxygen Species; 3317-61-1/5,5-dimethyl-1-pyrroline-1-oxide; 3352-57-6/Hydroxyl Radical

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