Document Detail


Detection of hydrogen gas-producing anaerobes in refuse-derived fuel (RDF) pellets.
MedLine Citation:
PMID:  16306688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, we reported that refuse-derived fuel (RDF) pellets contain a relatively high number of viable bacterial cells and that these bacteria generate heat and hydrogen gas during fermentation under wet conditions. In this study we analyzed bacterial cell numbers of RDF samples manufactured with different concentrations of calcium hydroxide, which is usually added to waste materials for the prevention of rotting of food wastes and the acceleration of drying of solid wastes, and determined the amount of hydrogen gas produced by them under wet conditions. Furthermore, we analyzed microflora of the RDF samples before and during fermentation by denaturing gradient gel electrophoresis of 16S rDNA followed by sequencing. We found that the RDF samples contained various kinds of clostridia capable of producing hydrogen gas.
Authors:
Makiko Sakka; Tetsuya Kimura; Kunio Ohmiya; Kazuo Sakka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  69     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-24     Completed Date:  2006-02-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2081-5     Citation Subset:  IM    
Affiliation:
Faculty of Bioresources, Mie University, Japan. makiko@bio.mie-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Bacteria, Anaerobic / isolation & purification,  metabolism*
Calcium Hydroxide
Colony Count, Microbial
DNA, Ribosomal
Fermentation
Hydrogen / metabolism*
Refuse Disposal / methods*
Chemical
Reg. No./Substance:
0/DNA, Ribosomal; 1305-62-0/Calcium Hydroxide; 1333-74-0/Hydrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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