Document Detail


Detection of a human parvovirus intrauterine infection with the polymerase chain reaction.
MedLine Citation:
PMID:  10845175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To detect human parvovirus B19 intrauterine infection in pregnancy with the polymerase chain reaction (PCR). STUDY DESIGN: DNA of chorionic villi and amniotic fluid was extracted and the gene of human parvovirus B19 amplified with PCR. RESULTS: The study analyzed 61 specimens of chorionic villi and 26 specimens of amniotic fluid and found two positive specimens of chorionic villi and 1 positive specimen of amniotic fluid. CONCLUSION: The vertical transmission of human parvovirus B19 infection in early pregnancy may be a pathway of intrauterine infection. Chorionic villus sampling in early pregnancy and PCR could be developed as a method of prenatal diagnosis of human parvovirus B19 intrauterine infection.
Authors:
Z W Dong; S Y Zhou; Y Li; R M Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of reproductive medicine     Volume:  45     ISSN:  0024-7758     ISO Abbreviation:  J Reprod Med     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-09-20     Completed Date:  2000-09-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0173343     Medline TA:  J Reprod Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  410-2     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, National Research Institute for Family Planning, Beijing, People's Republic of China. zwdong@ihw.com.cn
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MeSH Terms
Descriptor/Qualifier:
Adult
Amniotic Fluid / virology
Chorionic Villi / virology
DNA Primers
DNA, Viral / analysis*,  isolation & purification
Female
Humans
Infectious Disease Transmission, Vertical
Parvoviridae Infections / transmission,  virology*
Parvovirus B19, Human / genetics,  isolation & purification*
Polymerase Chain Reaction / standards*
Predictive Value of Tests
Pregnancy
Pregnancy Complications, Infectious / virology*
Prenatal Diagnosis / standards*
Uterine Diseases / virology*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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