Document Detail


Detection of human papillomavirus DNA in penile lesions histologically negative for condylomata. Analysis by in situ hybridization and the polymerase chain reaction.
MedLine Citation:
PMID:  2167614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to analyze penile lesions that lacked the histological features of condylomata for human papillomavirus (HPV) DNA. Most of these lesions were detected in men whose partners had an HPV-related lesion. Sequences homologous to HPV DNA were detected in 12 of 84 (14%) lesions using in situ hybridization analysis; nine of these lesions contained HPV 6 or 11. HPV DNA was found in 25 of 26 (96%) penile condylomata, all but two of which contained HPV 6 or 11. Using the highly sensitive polymerase chain reaction (PCR), we also detected HPV DNA in four of 20 (20%) of the penile lesions that lacked the histological features of condylomata negative for HPV by in situ analysis. Most of the lesions that were identifiable only after the application of an acetic acid solution were HPV negative when tested with either technique. We conclude that HPV DNA can be found in penile biopsy specimens that do not demonstrate the unequivocal histological features of condylomata but that the detection rate is much lower than for condylomata even when analyzing tissues in men at high risk for HPV-related lesions by PCR. This study underscores the need for caution when interpreting such tissues and the usefulness of in situ analysis for detecting assumed viral proliferation in noncondylomatous lesions.
Authors:
G J Nuovo; H A Hochman; Y D Eliezri; D Lastarria; S L Comite; D N Silvers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of surgical pathology     Volume:  14     ISSN:  0147-5185     ISO Abbreviation:  Am. J. Surg. Pathol.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-09-21     Completed Date:  1990-09-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7707904     Medline TA:  Am J Surg Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  829-36     Citation Subset:  IM    
Affiliation:
Department of Pathology, Columbia Presbyterian Medical Center, New York, New York.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Biopsy
Condylomata Acuminata / genetics*,  pathology
DNA Probes
DNA, Neoplasm / analysis*
DNA, Viral / analysis*
Humans
Male
Molecular Sequence Data
Nucleic Acid Hybridization
Papillomaviridae / genetics
Penile Neoplasms / genetics*,  pathology
Polymerase Chain Reaction
Sequence Homology, Nucleic Acid
Tumor Virus Infections / genetics*,  pathology
Chemical
Reg. No./Substance:
0/DNA Probes; 0/DNA, Neoplasm; 0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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