| Detection of high molecular weight proteins by MALDI imaging mass spectrometry. | |
| | |
MedLine Citation:
|
PMID: 23340489 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
MALDI imaging mass spectrometry (IMS) is a unique technology to explore the spatial distribution of biomolecules directly on tissues. It allows the in situ investigation of a large number of small proteins and peptides. Detection of high molecular weight proteins through MALDI IMS still represents an important challenge, as it would allow the direct investigation of the distribution of more proteins involved in biological processes, such as cytokines, enzymes, neuropeptide precursors and receptors. In this work we compare the traditional method performed with sinapinic acid with a comparable protocol using ferulic acid as the matrix. Data show a remarkable increase of signal acquisition in the mass range of 20k to 150k Th. Moreover, we report molecular images of biomolecules above 70k Th, demonstrating the possibility of expanding the application of this technology both in clinical investigations and basic science. |
| | |
Authors:
|
Veronica Mainini; Giorgio Bovo; Clizia Chinello; Erica Gianazza; Marco Grasso; Giorgio Cattoretti; Fulvio Magni |
Related Documents
:
|
11328839 - Observer accuracy in estimating proportions in images: implications for the semiquantit... 9336409 - Histologic/histochemical grading system for osteoarthritic articular cartilage: reprodu... 23085689 - Three-dimensional raman spectroscopic imaging of protein crystals deposited on a nanodr... 22398009 - Atrial isomerism: a pictorial review. 22741089 - In situ three-dimensional monitoring of collagen fibrillogenesis using shg microscopy. 19910219 - Does clinical pretest probability influence image quality and diagnostic accuracy in du... 15912589 - The role of global and feature based information in gender classification of faces: a c... 21946129 - Automatic detection of stent struts with thick neointimal growth in intravascular optic... 15835349 - Low-cost, scalable laser scanning module for real-time reflectance and fluorescence con... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-23 |
Journal Detail:
|
Title: Molecular bioSystems Volume: - ISSN: 1742-2051 ISO Abbreviation: Mol Biosyst Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101251620 Medline TA: Mol Biosyst Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Health Science, University of Milano-Bicocca, Monza, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Structural diversity among copper(i) ethylene adducts of 3,6-bis(2-pyridyl)-1,2,4,5-tetrazine.
Next Document: Conformational equilibria in carboxylic acid bimolecules: a rotational study of acrylic acid-formic ...