Document Detail

Detection of high molecular weight proteins by MALDI imaging mass spectrometry.
MedLine Citation:
PMID:  23340489     Owner:  NLM     Status:  Publisher    
MALDI imaging mass spectrometry (IMS) is a unique technology to explore the spatial distribution of biomolecules directly on tissues. It allows the in situ investigation of a large number of small proteins and peptides. Detection of high molecular weight proteins through MALDI IMS still represents an important challenge, as it would allow the direct investigation of the distribution of more proteins involved in biological processes, such as cytokines, enzymes, neuropeptide precursors and receptors. In this work we compare the traditional method performed with sinapinic acid with a comparable protocol using ferulic acid as the matrix. Data show a remarkable increase of signal acquisition in the mass range of 20k to 150k Th. Moreover, we report molecular images of biomolecules above 70k Th, demonstrating the possibility of expanding the application of this technology both in clinical investigations and basic science.
Veronica Mainini; Giorgio Bovo; Clizia Chinello; Erica Gianazza; Marco Grasso; Giorgio Cattoretti; Fulvio Magni
Related Documents :
18515509 - Interobserver agreement in the assessment of components of colposcopic grading.
23456419 - A feasible add-on upgrade on a commercial two-photon flim microscope for optimal flim-f...
24086059 - Purification of functionalized quantum dots.
19788739 - Intra- and inter-observer analysis in the morphological assessment of early-stage embryos.
9336409 - Histologic/histochemical grading system for osteoarthritic articular cartilage: reprodu...
24597479 - Label-free, all-optical detection, imaging and tracking of a single protein.
21308829 - Fetal second trimester four chamber view images evaluation via the internet: a comparis...
19776809 - Simultaneous raman scattering and laser-induced fluorescence for multispecies imaging i...
25163009 - Leaf gas exchange and chlorophyll a fluorescence imaging of rice leaves infected with m...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-23
Journal Detail:
Title:  Molecular bioSystems     Volume:  -     ISSN:  1742-2051     ISO Abbreviation:  Mol Biosyst     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101251620     Medline TA:  Mol Biosyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Health Science, University of Milano-Bicocca, Monza, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Structural diversity among copper(i) ethylene adducts of 3,6-bis(2-pyridyl)-1,2,4,5-tetrazine.
Next Document:  Conformational equilibria in carboxylic acid bimolecules: a rotational study of acrylic acid-formic ...