Document Detail

Detection of hepatitis B virus transcripts in patients with chronic liver disease.
MedLine Citation:
PMID:  2332589     Owner:  NLM     Status:  MEDLINE    
Hepatitis B virus (HBV) transcription was studied by Northern blot analysis on total cellular RNA purified from liver biopsies in 70 patients with chronic liver disease (24 HBsAg positive, 15 antiHBs and/or antiHBc positive, 31 HBV negative). No transcripts were found in the HBV negative and in the antiHBs and/or antiHBc positive patients. In the others, three major RNA species were identified: i. a 3.5 kb transcript corresponding to the RNA pregenome; ii. 2.4-2.1 kb transcript corresponding to the s and preS1 gene RNA; iii. lower molecular weight species. All three forms were present simultaneously only in patients with active viral replication, with a strict relation between the presence of the 3.5 kb RNA in the liver and serum HBV-DNA. In conclusion, Northern blot analysis can easily be performed to study viral replication and it can contribute to a better understanding of the molecular processes underlying HBV infection and leading to liver disease in man.
C Pasquinelli; M Melegari; E Villa; M Seidenari; P P Scaglioni; C Tiribelli; L S Crocé; F Manenti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of hepatology     Volume:  10     ISSN:  0168-8278     ISO Abbreviation:  J. Hepatol.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-06-07     Completed Date:  1990-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  180-5     Citation Subset:  IM    
Chair of Gastroenterology, University of Modena, Italy.
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MeSH Terms
Blotting, Northern
Chronic Disease
DNA Probes
DNA, Viral / isolation & purification
Hepatitis B Surface Antigens / analysis
Hepatitis B virus / genetics*,  isolation & purification
Liver Diseases / microbiology*
RNA, Viral / isolation & purification
Transcription, Genetic / physiology*
Reg. No./Substance:
0/DNA Probes; 0/DNA, Viral; 0/Guanidines; 0/Hepatitis B Surface Antigens; 0/Phenols; 0/RNA, Viral; 108-95-2/Phenol; 113-00-8/Guanidine

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