Document Detail


Detection of hTERT mRNA in gastrointestinal tract cancer specimens.
MedLine Citation:
PMID:  18564721     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human telomerase consisting of telomerase RNA template (hTR) and telomerase reverse transcriptase (hTERT) provides a mechanism for synthesis of telomere repeats that prolongs life span of cells. Telomerase activity is present in germ-line and malignant tumor cells but not in most normal human somatic cells. This study determined hTERT mRNA level in tissue samples from patients with gastrointestinal tract (GI) cancers. Tissue samples were obtained from 22 GI cancer patients, 3 gastrointestinal stomal tumors (GIST) and 25 corresponding non-cancerous tissues. hTERT expression was determined by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) using Taqman probe, hTERT mRNA was detected in 12 of 22 cancerous tissue samples. Six of 8 tissue samples obtained from patients with hepatocellular carcinoma and cholangiocarcinoma were positive for hTERT. However, hTERT mRNA was not detected in GIST and non-cancerous tissues. These results suggest that hTERT may be an effective target for cancer therapies to treat many type of GI cancers including cholangiocarcinoma and hepatocellular carcinoma.
Authors:
Wandee Udomchaiprasertkul; Siriluk Narong; Mesayamas Kongsema; Kawin Leelawat
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Southeast Asian journal of tropical medicine and public health     Volume:  39     ISSN:  0125-1562     ISO Abbreviation:  Southeast Asian J. Trop. Med. Public Health     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-06-20     Completed Date:  2008-12-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0266303     Medline TA:  Southeast Asian J Trop Med Public Health     Country:  Thailand    
Other Details:
Languages:  eng     Pagination:  324-7     Citation Subset:  IM    
Affiliation:
Cancer Molecular Therapeutics Unit, Chulabhorn Cancer Center, Chulabhorn Research Institute, Bangkok, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Hepatocellular / enzymology,  genetics*
Cholangiocarcinoma / enzymology,  genetics*
Gastrointestinal Neoplasms / enzymology,  genetics*
Humans
RNA / metabolism*
Telomerase / metabolism*
Chemical
Reg. No./Substance:
0/telomerase RNA; 63231-63-0/RNA; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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