Document Detail


Detection of gliadin in food using a quartz crystal microbalance biosensor that incorporates gold nanoparticles.
MedLine Citation:
PMID:  22694361     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This work develops a label-free gliadin immunosensor that is based on changes in the frequency of a quartz crystal microbalance (QCM) chip. A higher sensitivity was obtained by applying 25 nm gold nanoparticles (AuNPs) to the surface of a bare QCM electrode. Subsequently, chicken anti-gliadin antibodies (IgY) were immobilized directly on the AuNP-modified surface by crosslinking amine groups in IgY with glutaraldehyde. Experimental results revealed that the change in frequency exhibited when 2 ppm gliadin was bound to the AuNP-modified electrode was 35 Hz (48%) greater than that of the bare gold electrode. The linear dynamic range in 60% ethanol was from 1×101 to 2×105 ppb gliadin and the calculated limit of detection (LOD) was 8 ppb. The entire detection process was completed in 40 minutes and was highly repeatable. Additionally, the AuNP-modified QCM system generated results in the detection of gliadin in ten commercial food products that were consistent with those obtained using an AOAC-approved gliadin kit. In conclusion, the QCM platform provides a potential alternative means of ensuring that people with wheat allergies and celiac patients have access to gliadin-free food.
Authors:
Pei Tzu Chu; Chih-Sheng Lin; Wei-Jung Chen; Chih-Feng Chen; Hsiao-Wei Wen
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-14
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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