Document Detail


Detection and genotyping of GBV-C/HGV variants in China.
MedLine Citation:
PMID:  11172917     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We detected GBV-C/HGV sequences in the sera from 64 out of a total of 324 subjects in the south of China. In agreement with findings of others, we noted an especially high rate of infection among intravenous drug addicts and patients with chronic hepatitis C virus infection. The detection was achieved by nested PCR to amplify the 5' noncoding region (5'NCR) of the viral genome. Sequence analysis of the resulting 234 bp product revealed a total of 26 different sequences of which 25 were found to belong to the genotype G3, which is the most prevalent genotypes among Asian isolates, and one belonged to genotype G1, common among African isolates. The sequence divergence between the genotypes was largely clustered in a short variable region (V2) within the 5'NCR, and we showed that genotyping may be achieved equally well by analysis of this variable region as by the more detail analysis of the entire 5'NCR or of the entire viral genome.
Authors:
L Lu; M H Ng; B Zhou; H Luo; T Nakano; B H Robertson; S W Im
Related Documents :
11972417 - Genotyping of enterocytozoon bieneusi in aids patients from the north west of england.
17111177 - First report of cryptosporidium parvum 'ferret' genotype in american mink (mustela viso...
15184427 - Characterization of chlamydia trachomatis omp1 genotypes detected in eye swab samples f...
8799217 - Co-infection with two jc virus genotypes in brain, cerebrospinal fluid or urinary tract...
2691337 - Cloning and characterisation of the serc and aroa genes of yersinia enterocolitica, and...
21777247 - Analysis of two abundant, highly related satellites in the allotetraploid nicotiana are...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  73     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-08-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  131-44     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University Pathology Building, The University of Hong Kong, Queen Mary Hospital Compound, Pokfulam Road, Hong Kong.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
5' Untranslated Regions / genetics
Base Sequence
China / epidemiology
Cloning, Molecular
DNA, Complementary / genetics
Flaviviridae / classification,  genetics*,  isolation & purification
Genetic Variation / genetics*
Genotype
Hepatitis, Viral, Human / epidemiology,  virology*
Humans
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction / methods
RNA, Viral / blood
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/5' Untranslated Regions; 0/DNA, Complementary; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Differences in inhibition of replication between Coxsackie B4 virus strains in various cell lines by...
Next Document:  Mapping of linear epitopes on fibre knob of human adenovirus serotype 5.