| Detection of free radicals produced from reactions of lipid hydroperoxide model compounds with Cu(II) complexes by ESR spectroscopy. | |
| | |
MedLine Citation:
|
PMID: 8554344 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin trap, alkoxyl radicals and peroxyl radicals produced from the reactions of tert-butyl hydroperoxide(tBuOOH) and cumene hydroperoxide (PhC(CH3)2OOH) with some copper(Cu)(II) complexes such as Cu(II) complexes of cimetidine (Cim), cyclo(L-histidyl-L-histidyl) (CyHH), L-histidylglycine (HG), and L-histidylglycylglycine (HGG) were detected by electron spin resonance (ESR) spectroscopy. However, Cu(II) complexes of glycyl-L-histidine (GH), glycyl-L-histidylglycine (GHG),glycylglycyl-L-histidine (GGH), and glycylglycyl-L-histidyl-glycine (GGHG) did not cause the generation of free radicals during the reaction with tert-butyl or cumene hydroperoxide. Addition of a biological reductant such as cysteine or glutathione to the system including these Cu(II) complexes and hydroperoxides gave tert-butoxyl and cumyl alkoxyl (RO.) radicals, respectively. These alkoxyl radicals underwent subsequent beta-scission reaction and generated the carbon-centered radical (R.). Although cysteine and glutathione are considered to be cellular antioxidants, our results suggest that these biological reductants facilitate Cu(II) complexes-dependent free radical generation. |
| | |
Authors:
|
J Ueda; N Saito; T Ozawa |
Related Documents
:
|
16504514 - Quantitative 31p nmr detection of oxygen-centered and carbon-centered radical species. 21795804 - Overexpression, crystallization, and preliminary x-ray crystallographic analysis of shi... 18958324 - Chlorochromate ion as a catalyst for the photodegradation of chloroform by visible light. 9506944 - Rna folding at millisecond intervals by synchrotron hydroxyl radical footprinting. 8276784 - Crystal structure of rnase t1 with 3'-guanylic acid and guanosine. 16270954 - A tetranuclear cr(iii)ni(ii)3 cyano-bridged complex based on m(tacn) derivative buildin... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Archives of biochemistry and biophysics Volume: 325 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1996 Jan |
Date Detail:
|
Created Date: 1996-02-16 Completed Date: 1996-02-16 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 65-76 Citation Subset: IM |
Affiliation:
|
National Institute of Radiological Sciences, Chiba-shi, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alcohols
/
analysis Amino Acid Sequence Benzene Derivatives / chemistry Cimetidine / chemistry Copper / chemistry* Cyclic N-Oxides Electron Spin Resonance Spectroscopy* Free Radicals Histidine / chemistry Lipid Peroxides / chemistry* Molecular Sequence Data Oligopeptides / chemistry Peroxides / analysis, chemistry Spin Labels tert-Butylhydroperoxide |
| Chemical | |
Reg. No./Substance:
|
0/Alcohols; 0/Benzene Derivatives; 0/Cyclic N-Oxides; 0/Free Radicals; 0/Lipid Peroxides; 0/Oligopeptides; 0/Peroxides; 0/Spin Labels; 0/alkoxyl radical; 3170-83-0/perhydroxyl radical; 3317-61-1/5,5-dimethyl-1-pyrroline-1-oxide; 51481-61-9/Cimetidine; 71-00-1/Histidine; 7440-50-8/Copper; 75-91-2/tert-Butylhydroperoxide; 80-15-9/cumene hydroperoxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Thermal stability of horse spleen apoferritin and human recombinant H apoferritin.
Next Document: Differential effects of bovine PA28 on six peptidase activities of the lobster muscle proteasome (mu...