| Detection of free radicals in living mice after inhalation of DTBN by X-band ESR. | |
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MedLine Citation:
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PMID: 8384585 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new application of X-band electron spin resonance (ESR) is demonstrated for in vivo investigations on living mice. It is the first report on in vivo detection, identification, and localization of a free radical after uptake in the body of a living mammal by inhalation. The volatile di-tert-butyl-nitroxyl (DTBN) has been used as a suitable spin probe. After inhalation of DTBN, a superimposed ESR spectrum consisting of a polar (g = 2.0057; a = 1.65 mT) and a nonpolar paramagnetic species (g = 2.0061; a = 1.51 mT) was detected in mice tails. Collected blood exhibits only the polar spectrum. The decay of signals after removing of the DTBN-soaked swab follows first-order kinetics with k = 0.08 min-1. |
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Authors:
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K Mäder; R Stösser; H H Borchert |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Free radical biology & medicine Volume: 14 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 1993 Mar |
Date Detail:
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Created Date: 1993-04-23 Completed Date: 1993-04-23 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 339-42 Citation Subset: IM |
Affiliation:
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Department of Pharmacy, Humboldt University, Berlin, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Inhalation Animals Butanes Electron Spin Resonance Spectroscopy / methods* Free Radicals / analysis*, metabolism Male Mice Nitrogen Oxides / administration & dosage, pharmacokinetics Spin Labels Tail |
| Chemical | |
Reg. No./Substance:
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0/Butanes; 0/Free Radicals; 0/Nitrogen Oxides; 0/Spin Labels; 2406-25-9/di-t-butyl nitroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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